Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1998-01-22
2000-01-25
Mosher, Mary E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 693, 4353201, 4352523, 435325, 4353171, 536 234, 536 235, 530350, C12N 1509, C12N 1562, C12N 510, C07K 1900
Patent
active
060177357
ABSTRACT:
Coupled polypeptides and fusion polypeptides for intracellular transport, and their preparation and use, include (i) an aminoacid sequence with the transport function of herpesviral VP22 protein (or a homologue, e.g. from VZV, BHV or MDV) and (ii) another protein sequence selected from (a) proteins for cell cycle control; (b) suicide proteins; (c) antigenic sequences or antigenic proteins from microbial and viral antigens and tumour antigens; (d) immunomodulating proteins; and (e) therapeutic proteins. The coupled proteins can be used for intracellular delivery of protein sequences (ii), to exert the corresponding effector function in the target cell, and the fusion polypeptides can be expressed from corresponding polynucleotides. vectors and host cells.
REFERENCES:
Mercer et al., Wide type human p53 is antiproliferative in SV40-transformed hamster cells, Oncogene, vol. 5, pp. 973-980 (1990).
Shaw et al., Induction of apoptosis by wild-type p53 in human colon tumor-derived cell line, Proc. Natl. Acad. Sci., vol. 89, pp. 4495-4499 (1992).
Baker et al., Suppression of Human Colorectal Carcinoma Cell Growth by Wild-Type p53, Science, vol. 249, pp. 912-915 (1990).
Nicholson et al., "Localization of the herpes simplex virus type 1 major capside protein VP5 to cell nucleus requires the abundant scaffolding protein VP22a, " Journal of General Virology, vol. 75, No. 5, pp. 1091-1099 (1994).
Elliott et al., "VP16 interacts via its activation domain with VP22,a tegument protein of herpes simplex virus, and is relocated to a novel macromolecular assembly in coexperessing cells," Journal of Virology, vol. 69(12), pp. 7932-7941 (1995).
Elliott & O'Hare, "Intercellular Trafficking and Protein Delivery by a Herpesvirus Structural Protein," Cell, vol. 88, pp. 223-233 (1997).
Leslie, J. et al. Virology 220: 60-68, 1996.
Elliott Gillian Daphne
O'Hare Peter Francis Joseph
Marie Curie Cancer Care
Mosher Mary E.
LandOfFree
Materials and methods for intracellular transport and their uses does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Materials and methods for intracellular transport and their uses, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Materials and methods for intracellular transport and their uses will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2315018