Materials and methods for identifying genes and/or agents...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S254200, C435S254210, C435S254220

Reexamination Certificate

active

07618774

ABSTRACT:
Recombinant materials, including DNA constructs, expression vectors, and host cells, and methods of identifying an environmental stimulus or a gene that alters the lifespan of an organism are identified. The recombinant DNA constructs include first and second chimeric genes that both encode substantially the same protein that is required for yeast replication, the first chimeric gene containing a promoter responsive to growth medium conditions and the second chimeric gene containing a promoter operable in mother cells but not daughter cells.

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Minois, N. How Should We Assess the Impact of Genetic Changes on Ageing in Model Species? Ageing Research Reviews 5:52-59, 2006.
Jarolim, S. et al. A novel assay for replicative lifespan inSaccharomyces cerevisiaeFEMS Yeast Research 5:169-177, 2004.
Bobola et al., “Asymmetrical Accumulation of Ash1p in Postanaphase Nuclei Depends on a Myosin and Restricts Yeast Mating-Type Switching to Mother Cells,”Cell84:699-709 (1996).
Jarolim et al., “Is There a Way to Select For Long Lived Yeast Mutants?” at 1stInternational Meeting of Yeast Apoptosis, Braga, Portugal (Oct. 4-6, 2002) retrieved from the internet at http://www.bio.uminho.pt/imya/html/ab8.htm.

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