Materials and methods for herpes simplex virus vaccination

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence

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530326, 424 89, C07K 706, C07K 708, A61K 3912

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active

047090111

ABSTRACT:
Disclosed are immunologically active preparations of Herpes simplex virus envelope glycoproteins, gD-1 and gD-2. Preferably purified through use of a monoclonal anti-gD antibody immunodsorbent, gD-1 and gD-2 preparations are incorporated in vaccines useful in generating immunological responses protective against Herpes simplex virus disease states. Disclosed also is the preparation and use in vaccination procedures of synthetic polypeptides comprising amino acid squences which are: (1) substantially common to both gD-1 and gD-2; (2) cumulatively hydrophilic in nature; (3) specifically immunoreactive with a type common, monoclonal anti-gD antibody of Group VII classification. Vaccines incorporating the synthetic polypeptides give rise to protective immunological responses, e.g., they protect mice against Herpes simplex.

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Watson et al., Science, vol. 218, pp. 381-384, (1982).
Cappel, Archives of Virology, vol. 52, pp. 29-35 (1976).
Slichtova, Archives of Virology, vol. 66, pp. 207-214 (1980).
Kutinova et al., Archives of Virology, vol. 61, pp. 141-147 (1979).
Klein et al., Archives of Virology, vol 68, pp. 73-80 (1981).
Kitces et al., Infect. Immun., 16, pp. 955-960 (1977).
Norrild, Current Topics in Microbiology and Immunology, vol. 90, pp. 67-106, (1980).
Lasky et al., DNA, 3, 23-29, 1984.

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