Materials and methods for digestion of DNA or RNA using restrict

Details Materials and methods for digestion of DNA or RNA using restrict Materials and methods for digestion of DNA or RNA using restrict

1995-06-07

1999-03-09

Horlick, Kenneth R.

Chemistry: analytical and immunological testing

Including sample preparation

Digestion or removing interfering materials

436176, 436177, 436178, 435262, 435264, 435 681, 435 691, 435 692, 435 41, 435182, 435183, 435195, 435814, 4351734, 2057775, G01N 100, G01N 118, C07C 102, C07D 102

Patent

active

058799508

ABSTRACT:
Disclosed are improvements for enzyme-catalyzed reactions involving DNA or RNA, including restriction digests, which are based on conducting such reactions in the presence of lipids.

REFERENCES:
New England Biolabs Catalog, p. 196, 1995.
Batzri et al. "Single bilayer liposomes prepared without sonication". Biochemica et Biophysica Acta., vol. 298, pp. 1015-1019, 1973.
Heitman, "On the Origins, Structures and Functions of Restriction-Modification Enzymes," Genetic Engineering, 15:57-108, Plenum Press, New York (1993).
Heitman, "SOS induction as an in vivo assay of enzyme -DNA interactions," Gene, 103:1-9 (1991).
Kere, et al., "Mapping Human Chromosomes by Walking with Sequence-Tagged Sites from End Fragments of Yeast Artificial Chromosome Inserts," Genomics, 14:241-248 (1992).
Budker, et al., Chemical Abstracts, 116:152013b (1987).
Mixich, "Induction of chromosomal aberrations by restriction endonucleases encapsulated in liposomes," Mutation Research, 262:177-181 (1991).
Bryant, et al., "Induction of chromosomal aberrations in CHO cells by restriction endonucleases: effects of blunt-and cohesive-ended double-strand breaks in cells treated by `pellet` methods," Mutation Research, 213:233-241 (1989).
Obe, et al., "Chromosomal aberrations induced by restriction endonucleases," Mutation Research, 150:359-368 (1985).

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