Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Patent
1997-06-26
2000-07-18
Riley, Jezia
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
435 6, 435 911, 435 912, 536 221, 536 241, 536 2433, 536 237, 530350, C12Q 168, C12N 1500, C07H 1900, C07K 100
Patent
active
060906286
ABSTRACT:
The subject invention concerns the novel use of formyl-CoA transferase enzyme together with oxalyl-CoA decarboxylase enzyme for the detection and measurement of oxalate in biological samples. The use of the enzyme system according to the subject invention results in the conversion of oxalate into carbon dioxide and formate. Because the production of formate is directly correlated to the concentration of oxalate present in a sample, the determination of the resulting formate concentration provides an accurate, sensitive and rapid means for detecting even low levels of oxalate. The subject invention further concerns the cloning, sequencing and expression of the genes that encode the formyl-CoA transferase enzyme and the oxalyl-CoA decarboxylase enzyme of Oxalobacter formigenes. The subject invention also concerns methods for detecting the presence of Oxalobacter formigenes organisms in a sample, and the polynucleotide probes and primers used in the detection method.
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Peck Ammon B.
Sidhu Harmeet
Riley Jezia
University of Florida
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