Materials and methods for amplifying polynucleotides in plants

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 691, 435 911, 4353201, 435411, 435414, 435417, 435419, 800278, 800288, 800298, 8003172, 8003173, 8003174, 536 236, C12N 504, C12N 1529, C12N 1569, C12N 1590, C12N 1582

Patent

active

061000922

ABSTRACT:
Products and methods for amplifying target nucleic acids using cells derived from plants are disclosed. The products include nucleic acids containing a plant active Amplification Promoting Sequence (APS) and the methods exploit these products in amplifying target nucleic acids. Also disclosed are methods for amplifying target nucleic acids that express an encoded product, and the recovery of that expression product. The methods of the invention minimize operator intervention and exploit solar energy and the minimal nutrient needs of photoautotrophic organisms to provide inexpensive and indefinitely sustainable methods for producing a variety of amplified target nucleic acids and encoded products such as polypeptides.

REFERENCES:
patent: 5106739 (1992-04-01), Comai et al.
Gruendler et al, J. Mol. Biol., vol. 221, pp. 1209-1222, 1991.
Borisjuk et al, Plant Mol. Biol., vol. 35, pp. 655-660, 1997.
Perry et al, Mol. Gen. Genet., vol. 221, pp. 102-112, 1990.
Borisjuk et al, Plant Mol. Biol., vol. 21, pp. 381-384, 1993.
Hernandes et al, EMBO J., vol. 12, pp. 1475-1485, 1993.
Odell et al, Mol. Gen. Genet., vol. 223, pp. 369-378, 1990.
Borisjuk, N.V. et al., "Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa," Theor. Appl. Genet., 76:108-112 (1988).
Borisjuk, N.V. et al., "Structural analysis of rDNA in the genus Nicotiania," Plant Molecular Biology, 35:655-660 (1997).
Christou, P., "Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment," Euphytica, 85:13-27 (1995).
Doelling, J.H. et al., "Functional analysis of Arabidopsis thaliana rRNA gene and spacer promoters in vivo and by transient expression," Proc. Natl. Acad. Sci. USA, 90:7528-7532 (Aug. 1993).
Doelling, J.H. et al., "The minimal ribosomal RNA gene promoter of Arabidopsis thaliana includes a critical element at the transcription initiation site," The Plant Journal, 8(5):683-692 (1995).
Fan, H. et al., "In vitro transcription of plant RNA polymerase I-dependent rRNA genes is species-specific," The Plant Journal, 8(2):295-298 (1995).
Gruendler, P. et al., "rDNA Intergenic Region from Arabidopsis thaliana: Structural Analysis, Intraspecific Variation and Functional Implications," J. Mol. Biol., 221:1209-1222 (1991).
Hemann, C. et al., "High-Copy Expression Vector-Based on Amplification-Promoting Sequences," DNA and Cell Biology, 13(4):437-445 (1994).
Holst, A. et al., "Murine Genomic DNA Sequences Replicating Autonomously in Mouse L Cells," Cell, 52:355-365 (1988).
Hood, E.E. et al., "The Hypervirulence of Agrobacterium tumefaciens A281 Is Encoded in a Region of pTiBo542 Outside of T-DNA," J. Bacteriol., 168(3): 1291-1301 (1986).
Jackson, S.D. et al., "Protein-binding to reiterated motifs within the wheat rRNA gene promoter and upstream repeats," Plant Molecular Biology, 20:911-919 (1992).
Jahne, A. et al., "Genetic engineering of cereal crop plants: a review," Euphytica, 85:35-44 (1995).
Kellems, R.E., "Gene Amplification Strategies for Protein Production in Mammalian Cells," Methods in Molecular Genetics, 5:143-155 (1994).
Lopes, T.S. et al., "High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression," Gene, 79:199-206 (1989).
Marilley, M. et al., "Common DNA structural features exhibited by eukaryotic ribosomal gene promoters," Nucleic Acids Research, 24(12):2204-2211 (1996).
Meyer, J. et al., "Inhibition of HIV-1 replication by a high-copy-number vector expressing antisense RNA for reverse transcriptase," Gene, 129:263-268 (1993).
Reichel, C. et al., "Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono-and dicotyledonous plant cells," Proc. Natl. Acad. Sci.(USA), 93:5888-5893 (1996).
Sambrook et al., .sctn..sctn.9.47-9.51 in Molecular Cloning: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York 1989).
Vain, P. et al., "Foreign Gene Delivery Into Monocotyledonous Species," Biotechnology Advances, 13(4): 653-671 (1995).
Wegner, M. et al., "Cis-acting sequences from mouse rDNA promote plasmid DNA amplification and persistence in mouse cells: implication of HMG-I in their function," Nucleic Acids Research, 17(23):9909-9932 (1989).
Zastrow, G. et al., "Distinct mouse DNA sequences enable establishment and persistence of plasmid DNA polymers in mouse cells," Nucleic Acids Research, 17(5):1867-1879 (1989).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Materials and methods for amplifying polynucleotides in plants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Materials and methods for amplifying polynucleotides in plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Materials and methods for amplifying polynucleotides in plants will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1149205

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.