Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Reexamination Certificate
1999-11-16
2001-05-15
Snay, Jeffrey (Department: 1743)
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
C204S450000, C204S600000, C204S601000
Reexamination Certificate
active
06231737
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates generally to the field of chemical analysis and testing and, more specifically, to methods for transporting materials processed on microchips to off-chip devices for further interrogation. A microchip generates an electrospray from solutions emerging from channels etched on planar substrates. Fluids are delivered through the channels using either hydraulic or electrically driven forces and are sprayed electrostatically from the terminus of the channel. An electrical potential difference of sufficient magnitude to generate the electrospray is applied between a location on the microchip and a conductor spaced from the terminus of the channel.
BACKGROUND OF THE INVENTION
Recently, miniaturized chemical instruments have been fabricated using micromachining techniques. These “microchip” devices have been used to perform liquid phase separations such as electrochromatography and electrophoresis and mixing of reagents in integrated microreactors for chemical reactions.
Among their many advantages, microchips allow increased speed of analysis and reduced reagent and sample consumption over conventional bench-scale instruments. In addition, integrated devices provide significant automation advantages as fluidic manipulations are computer controlled. These integrated devices are now being referred to as “lab on a chip” technologies, as the operations of a complete wet chemical laboratory could potentially be integrated.
Many chemical analysis tools that are used in modern laboratories are not presently miniaturized and many are not thought to be amenable to miniaturization in the immediate future. Although the microchip technology is quite powerful, there are situations where after processing on a microchip, further chemical interrogation is desired off of the microchip. A convenient way to transport fluids from microchips to other devices is to generate droplets that can be directed to specific locations for collection and/or analysis. Ink jet technologies are in wide spread use in printer products and are currently used to distribute liquid borne chemicals with spatial selectivity. Early ink jet formation methods included continuous stream ink jets, impulse ink jets, electrostatically generated ink jets. See, for example, R. D. Carnahan, and S. L. Hou,
IEEE Trans. Ind. Appl
., IA-13, 95 (1977). The first two methods use acoustic energy to form droplets and the latter uses electrostatic forces. Most modern ink jet printers utilize thermal energy to form droplets where bubble formation in the ink essentially provides the acoustic driving force to launch a droplet. See, for example, R. A. Askeland, W. D. Childers, and W. R. Sperry,
Hewlett Packard Journal
, August, pg. 28 (1988). Charged droplets and even molecular ions can be generated from liquids using the technique called “electrospray.”
Electrospray is often used as a method for generating gas phase ions from solution for subsequent mass spectral analysis. Electrospray ionization is a soft ionization technique whereby species that are ionic in solution are transferred to the gas phase. The sample solution is dispersed as an electrically-charged aerosol and following solvent evaporation and disintegration of the droplets into smaller droplets, gas-phase ions are eventually produced. In the past, electrospray ion sources have employed needles or capillary tubes for spraying ion sources.
Essentially no fragmentation accompanies the ionization process and multiply-charged ions are typically produced from high mass polymers such as peptides, proteins, DNA and various synthetic polymers. Thus, electrospray ionization mass spectrometry is an effective means to provide primary and secondary structural analysis of polymeric materials.
SUMMARY OF THE INVENTION
An object of the present invention is to provide a method and apparatus for electrospraying a material for analysis in which the speed of analysis in increased.
Another object of the present invention is to provide a method and apparatus for transporting material from microchips by generating droplets that are directed to a receiving substrate or device.
Another object of the present invention is to provide a method and apparatus for electrospraying a material for analysis in which the amount of reagent and sample consumption are reduced.
Another object of the present invention is to provide a method and apparatus for electrospraying a material for analysis which is cost effective to produce and relatively simple in construction.
Another object of the invention is to provide a method and apparatus for electrospraying which enables a material manipulated by a planar substrate to be electrosprayed directly to a receiving device which uses the material as a gas phase entity or collects the droplets.
These and other objects are achieved by providing a method of electrospraying a material which includes placing the material i n a channel of a microchip, and forming an electric field in the channel with sufficient magnitude and direction to cause the material to be sprayed from an outlet of the channel.
Other objects, advantages, and salient features of the invention will become apparent from the following detailed description, which taken in with the annexed drawings, discloses the preferred embodiment of the present invention.
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M. Deml, F. Foret, and P. Bo{haeck over (c)}ek, “Electric Sample Splitter for Capillary Electrophoresis” Journal of Chromatography, 320 No month available (1985) 159-165.
Ramsey J. Michael
Ramsey Roswitha S.
Dann Dorfman Herrell and Skillman, P.C.
Snay Jeffrey
Starsiak Jr. John S.
UT-Battelle LLC
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