Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1990-05-09
1991-04-23
Morgenstern, Norman
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435180, 427 2, 427 36, 427 531, 427337, 4273855, 525276, 623 1, 623 12, 623 15, C12N 502, A61K 4700
Patent
active
050100092
DESCRIPTION:
BRIEF SUMMARY
FIELD OF INVENTION
This invention relates to the chemical modification of polytetrafluoroethylene (PTFE, which is sold under the Registered Trade Mark TEFLON) and other fluorocarbon polymers, to produce a surface that is a suitable substratum for the attachment and growth of adherent animal cells.
The invention also relates to chemical procedures to achieve this modification of PTFE and other fluorocarbon polymers and to procedures for the preparation of the chemically modified surface for cell attachment. Particular attention is drawn to the ability of PTFE modified by these procedures to serve as a substratum for the attachment and growth of human endothelial cells, and so to the potential use of these chemically modified fluorocarbon polymers and procedures in the preparation of implantable materials including vascular prostheses and percutaneous implants.
BACKGROUND ART
Information gained during in vitro cell culture experiments can profitably be used in the design or selection of materials for use in specific biomaterials such as vascular prostheses. The attachment and growth of endothelial cells and other anchorage-dependent animal cells during in vitro cell culture requires both a suitable substratum for cell attachment and a culture medium that contains either serum, or certain purified serum proteins.
Concerning the chemical nature of the substratum, cells such as endothelial cells do not attach and grow well on hydrophobic surfaces such as nonwettable polystyrene (bacteriological plastic) or on PTFE which is commonly used in vascular prosthetic grafts. On the other hand, cells including endothelial cells also fail to adhere to many hydrophilic polymers, such as the hydrogel poly-2-hydroxyethylmethacrylate, polyHEMA. Cells do attach and grow on polymers where the surface is composed of microdomains containing both hydrophobic and hydrophilic regions. The use of polymers with a microdomain structure of this nature is now the state of the art in the biomedical material area (e.g. the polyurethanes sold under the Registered Trade Marks BIOMER and MITRATHANE). The perfluorosulphonate ionomer which is known by the Registered Trade Mark NAFION has recently been shown to be suitable for endothelial cell attachment and growth (International patent application PCT/AU88/00368; McAuslan et al., (1988) J. Biomed. Mater. Res., 22,963-976; Norris et al., (1988) Clinical Materials, 3,153-162; and may also fit this generalisation, in that as only 1 in every 8 monomer units is sulphonated, the large segments of uncharged chains may allow for both hydrophobic and hydrophilic interactions.
The surface that is commonly used for animal cell attachment and growth in vitro is polystyrene, modified by one of a number of techniques to produce a surface that can promote cell attachment (tissue culture polystyrene). This modification of polystyrene has been performed by treatment with sulphuric acid (Kennedy & Axelrod, (1971) Immunology, 20,253-257); with chromic acid or with sulphuric acid and chromic acid (Klemperer & Knox, (1977) Laboratory Practice 26(3), 179-180); or treatment with a corona discharge process (Maroudas, (1973) in "New Techniques in Biophysics and Cell Biology" (R. H. Payne and B. J. Smith, eds) Wiley Interscience, London). These treatments are believed to introduce hydroxyl groups, and the surface concentration of hydroxyl groups must fall within a range for the polystyrene derivative to be suitable for cell attachment (Curtis et. al., (1983) J. Cell Biology 97, 1500-1506; and Curtis et. al. (1986) J. Cell Sci. 86,9-24). Carboxyl groups produced in the reactions appear to play only a small role in the cell adhesion to modified polystyrene (Curtis et al., 1986). Very few sulphonate groups are introduced into the surface (Curtis et al. 1983).
Somewhat different results as to the surface groups required for cell attachment were obtained in a study of cell attachment to polyHEMA by McAuslan et al (PCT/AU87/00043 and McAuslan et al., J. Biomed. Mater. Res., 1987). In that study it was shown that hydrolyt
REFERENCES:
patent: 3839172 (1974-10-01), Chapiro et al.
patent: 4143218 (1979-03-01), Adams et al.
patent: 4743258 (1988-05-01), Ikada et al.
patent: 4877839 (1989-10-01), Conti-Ramsden et al.
patent: 4897433 (1990-01-01), Sugo et al.
Hodgkin Johnathon
Johansen Oddvar
Johnson Graham
Steele John
Commonwealth Scientific & Industrial Research Organisation &
Morgenstern Norman
Owens Terry J.
LandOfFree
Material for cell attachment and growth does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Material for cell attachment and growth, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Material for cell attachment and growth will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1621142