Material for affinity chromatography which is a sulfated polysac

Details Material for affinity chromatography which is a sulfated polysac Material for affinity chromatography which is a sulfated polysac

1990-07-16

1992-05-26

Brown, Johnnie R.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

530413, 536 187, 536 51, 536 21, C08B 3710, C08G 400

Patent

active

051169622

ABSTRACT:
A process for the preparation of a material for affinity chromatography is described, in which a sulfated polysaccharide is bonded to a carrier material which has amino groups.
For this purpose, a sulfated polysaccharide is treated with an oxidizing agent which oxidizes glycols to aldehydes, with cleavage of the carbon chain, and this modified sulfated polysaccharide is allowed to react with a carrier which has amino groups.
It is possible using the described affinity material to adsorb proteins which bind to sulfated polysaccharides from solutions of these proteins and, where appropriate, then to desorb them, for example antithrombin III from blood plasma.

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Andersson et al., Purification of Antithrombin III by Affinity Chromatography, vol. 5, No. 4, 1974, pp. 439-452.
Porath et al., Journal of Chromatography, 51, 1970, pp. 479-489.
Sundberg et al., Journal of Chromatography, 90, 1974, pp. 87-98.
Isamu et al., Journal of Chromatography, 239, 1982, pp. 747-754.

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