Mass spectroscopic method for comparing protein levels in...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C530S402000, C530S412000, C530S815000, C530S816000

Reexamination Certificate

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07635573

ABSTRACT:
The present invention provides a new and improved method for quantitative determination and/or identification of proteins in a sample. In one aspect, the invention provides a mass spectroscopic method for comparing protein levels in two or more samples by differentially isotopically labeling each sample's proteins' N- or C-termini. In another aspect, the invention provides a mass-spectroscopic method for identifying a sample as source for a protein from a mixture of two or more samples by differentially isotopically labeling each sample's proteins' N- or C-termini.

REFERENCES:
patent: 5240859 (1993-08-01), Aebersold
patent: 5538897 (1996-07-01), Yates et al.
patent: 5856082 (1999-01-01), Aebersold et al.
patent: 6064754 (2000-05-01), Parekh et al.
patent: 6225047 (2001-05-01), Hutchens et al.
patent: 6278794 (2001-08-01), Parekh et al.
patent: 6316267 (2001-11-01), Bhalgat et al.
patent: 6322970 (2001-11-01), Little et al.
patent: 6329209 (2001-12-01), Wagner et al.
patent: 6331400 (2001-12-01), Woods
patent: 6872575 (2005-03-01), Regnier
patent: 7052915 (2006-05-01), Aebersold et al.
patent: 7052916 (2006-05-01), Johnson
patent: 7122377 (2006-10-01), Qiu et al.
patent: 7332613 (2008-02-01), Gygi et al.
patent: 7422865 (2008-09-01), Fischer
patent: 2001/0039016 (2001-11-01), Waldman et al.
patent: 2002/0106700 (2002-08-01), Foote et al.
patent: WO00/11208 (2000-03-01), None
patent: WO01/96869 (2001-12-01), None
patent: WO02/77016 (2002-10-01), None
patent: WO02/97703 (2002-12-01), None
patent: WO02/97703 (2003-07-01), None
Aebersold et al., “Equipping Scientists for the New Biology,” Nat. Biotechnol., 2000, 18(4): 359.
Anderson et al., “Proteomics: Applications in Basic and Applied Biology,” Curr. Opin. Biotechnol., 2000, 11(4): 408-412.
Appella et al., “Meeting report. Methods in Protein Structural Analysis Conference (MPSA2000), Charlottesville, Virginia, Sep. 16-20, 2000,” Protein Sci., 2001, 10(2): 459-461.
Ashman et al., “Cell Signalling—The Proteomics of it All,” Sci. STKE, 2001, 2001(103):pe33.
Beardsley et al., “Enhancing the intensities of lysine-terminated tryptic peptide ions in matrix-assisted laser desorption/ionization mass spectrometry,” Rapid Commun. Mass Spectrom., 2000, 14(23): 2147-2153.
Cagney et al., “De Novo Peptide Sequencing And Quantitative Profiling Of Complex Protein Mixtures Using Mass-Coded Abundance Tagging,” Nat. Biotechnol, 2002, 20(2): 163-170.
Chich, “A Mini Review: Proteomic Analysis, a Post-Genomic Approach,” Le Lait, 2001, 81:13-18.
Dutt et al., “Proteomic Analysis,” Curr. Opin. Biotechnol., 2000, 11(2): 176-179.
Eng et al., “An Approach to Correlate Tandem Mass Spectral Data of Peptides with Amino Acid Sequences in a Protein Database”, J Am. Soc. Mass. Spectrom., 1994, 5: 976-989.
Ewing et al., “Peptide Fingerprinting on the OmniFLEX: Bench-Top Protein Identification,” Bruker Daltonics, Inc., pp. 1-6.
Gevaert et al. “Chromatographic isolation of methionine-containing peptides for gel-free proteome analysis: identification of more than 800Escherichia coliproteins,” Mol. Cell. Proteomics, 2002,1(11): 896-903.
Gevaert et al., “Exploring Proteomes and Analyzing Protein Processing by Mass Spectrometric Identification Of Sorted N-Terminal Peptides,” Nat. Biotechnol., 2003, 21(5): 566-569.
Gygi et al., “Measuring Gene Expression by Quantitative Proteome Analysis,” Curr. Opin. Biotechnol., 2000, 11(4): 396-401.
Keough et al., “A method for high-sensitivity peptide sequencing using postsource decay matrix-assisted laser desorption ionization mass spectrometry,” Proc. Natl. Acad. Sci. USA, 1999, 96(13):7131-7136.
Mann, “Quantitative Proteomics?” Nat. Biotechnol., 1999, 17(10): 954-955.
McCormack et al., “Direct analysis and identification of proteins in mixtures by LC/MS/MS and database searching at the low-femtomole level,” Anal. Chem., 1997, 69(4): 767-776.
Pandey et al., “Proteornics to Study Genes and Genomes,” Nature, 2000, 405(6788): 837-846.
Peters et al., “A Novel Multifunctional Labeling Reagent for Enhanced Protein Characterization with Mass Spectrometry,” Rapid Commun. Mass Spectrom., 2001, 15(24):2387-2392.
Rapp et al., “Detection Limits of MALDI-TOF PSD for Peptide Sequencing from Protein Digests,” Bruker Daltonics, 2000, pp. 1-6.
Shim et al., “PepAssem, A Peptide Assembly Algorithm for a Complex Protein Mixture,” Genome Inform., 2001, 12: 366-367.
Suckau et al., “Automation of MALDI-TOF Analysis for Proteomics,” Bruker Daltonics, 1999, pp. 1-5.
Suckau et al., “N-Terminal Derivatization of Tryptic Peptides for de Novo Sequencing by PSD: A Powerful New Tool in Proteomics,” Bruker Daltonics, 2000, pp. 1-4.
Swiderek et al., “The identification of peptide modifications derived from gel-separated proteins using electrospray triple quadrupole and ion trap analyses,” Electrophoresis, 1998, 19(6): 989-997.
Tang et al., “An Automated C-Terminal Sequencing Method for Analysis of Proteins Using an ABI 473A Sequencer,” J Biomol. Tech., 1999, 10: 144-148.
Wilkins et al., “Rapid Protein Identification Using N-Terminal “Sequence Tag” and Amino Acid Analysis,” Biochem. Biophys. Res. Commun., 1996, 221(3): 609-613.

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