Mass spectrometry-based methods for detection and...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Reexamination Certificate

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07611856

ABSTRACT:
The present invention is directed to a method for detecting the presence of clostridial neurotoxins in a sample by mixing a sample with a peptide that can serve as a substrate for proteolytic activity of a clostridial neurotoxin; and measuring for proteolytic activity of a clostridial neurotoxin by a mass spectroscopy technique. In one embodiment, the peptide can have an affinity tag attached at two or more sites.

REFERENCES:
patent: 5965699 (1999-10-01), Schmidt et al.
patent: 6504006 (2003-01-01), Shine et al.
patent: 6762280 (2004-07-01), Schmidt et al.
patent: 6803475 (2004-10-01), Wipf et al.
patent: WO03001206 (2003-01-01), None
patent: WO 03001206 (2003-01-01), None
Kalb et al. (Anal. Biochem., 351:84-92, 2006).
Boyer et al. (Anal. Chem., 77:3916-3924, 2005).
Schmidt et al., Anal. Biochem., 296:130-137, 2001.
Schmidt and Stafford (Appl. Environ. Microbiol., 69:297-303, 2003).
Hortin et al. (Clin. Chem., 47:215-222, 2001).
Bowers et al. (Anal. Chem., 65:475R-479R, 1993).
Schmidt et al., Appl. Microbiol., v69 (1) pp. 297-303 (2003).

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