Mass spectrometric analysis of proteins using stable...

Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...

Reexamination Certificate

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C436S086000, C436S089000, C436S090000

Reexamination Certificate

active

08071392

ABSTRACT:
The present invention provides a protein analysis method using a combination of two or more kinds of stable isotopes of a compound represented by the formula (I):wherein R1, R2and R3are the same or different and each is hydrogen, halogen or alkyl, or a salt thereof, as a labeling compound to produce a difference in the mass of the same kind of protein contained in each sample.

REFERENCES:
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patent: 1 696 230 (2006-08-01), None
patent: 2003-107066 (2003-04-01), None
patent: 2005-181011 (2005-07-01), None
patent: 2007-127631 (2007-05-01), None
patent: 00/11208 (2000-03-01), None
International Search Report issued Jul. 29, 2008 in International (PCT) Application No. PCT/JP2008/061243.
Douglas B. Craig et al., “Determination of picomolar concentrations of proteins using novel amino reactive chameleon labels and capillary electrophoresis laser-induced fluorescence detection”, Electrophoresis, vol. 26, Issue 11, pp. 2208-2213, May 9, 2005.
Ion Ghiviriga et al., “Rotation Barriers in Pyridinium Salts Depend on the Number of Available Ground State Conformations”, Croatica Chemica Acta, vol. 77, No. 1-2, pp. 391-396, 2004.
Michael B. Goshe et al., “Stable isotope-coded proteomic mass spectrometry”, Current Opinion in Biotechnology, vol. 14, No. 1, pp. 101-109, Feb. 2003.
Form PCT/IB/338 together with translation of International Preliminary Report on Patentability in International (PCT) Application No. PCT/JP2008/061243.

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