Mass spectrometric analysis of biopolymers

Details Mass spectrometric analysis of biopolymers Mass spectrometric analysis of biopolymers

2008-07-08

2008-07-08

Le, Long V. (Department: 1641)

Chemistry: analytical and immunological testing

Nuclear magnetic resonance, electron spin resonance or other...

C435S006120, C435S007920, C436S175000, C210S656000

Reexamination Certificate

active

07396688

ABSTRACT:
The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separation technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.

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