Mass-production method for seedling of seed potato

Multicellular living organisms and unmodified parts thereof and – Method of using a plant or plant part in a breeding process... – Method of breeding involving a tissue culture step

Reexamination Certificate

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Reexamination Certificate

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07906703

ABSTRACT:
Disclosed is a method of mass producing potato seedlings, comprising collecting growing points of seed potatoes and culturing the growing points in a liquid or solid medium; introducing in vitro plantlets obtained from the culture of the growing points to solid culture; and removing the in vitro plantlets from the solid culture, and planting through stem cutting and acclimatizing the in vitro plantlets in deep flow culture, in which a nutrient solution is circulating. Upon planting in hydroponic facilities, such potato seedlings have high adaptability to the external environment and thus rapidly, uniformly generate roots in a short time. The rapid root anchoring prevents planted seedlings from withering, leading to death, growing poorly, and the like. The direct planting of in vitro plantlets through stem cutting without a separate acclimatization process shortens the overall production period of potato seedlings by omitting the acclimatization process.

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Akita et al. “Stimulation of potato (Solanum tuberosumL.) tuberization by semi continuous liquid medium surface level control,” Plant Cell reports (1994) 13:184-187.
Jimenez et al. “Improved production of potato microtubers using a temporary immersion system,” Plant Cell. Tissue and Organ Culture 69: 19-23, 1999.

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