Mass production and long-term preservation of fungivorous nemato

Details Mass production and long-term preservation of fungivorous nemato Mass production and long-term preservation of fungivorous nemato

1998-05-12

2000-03-07

Saucier, Sandra E.

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

424 937, 435260, A01K 6300, C12N 104

Patent

active

060336579

ABSTRACT:
A host fungus of a fungivorous nematode is inoculated on a solid medium or an artificial liquid medium containing an industrial vegetable waste or by-product, and then the nematode whose whole body have been sterilized is inoculated and mass-cultivated. Fungivorous ability of the nematode can be kept by subculturing using different host fungus on every culturing stage. The nematodes, when maintained about 10 days in an aerobic condition at 20-25.degree. C. with a relative humidity gradually inclined from high to low and dried to anhydrobiotic conditions, can be preserved for a long time. The nematode can be used for biological control of soil pathogens and soil insect pests.

REFERENCES:
patent: 5183950 (1993-02-01), Popiel et al.
patent: 5554533 (1996-09-01), Bedding et al.
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Townshend, Fungus Hosts of Aphelenchus Avenae Bastian, and Bursaphelechus Fungivorus Franklun & Hooper, 1962 and their attractiveness to these nematode species, Canadian Journal of Microbiology, Mar. 24, 1964, pp. 727-737.
Choi et al. "Preparation of Five Aphelechus avenae Isolates on Six Species of Fungi and Five Substrates" Japan Journal of Nematology, vol. 19, Dec. 1989, pp. 13-17.
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