Mass and a method of preparing same of living cells of organisms

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435253, 435257, 435262, C12N 1300, C12N 120, C12N 112, C02F 300

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active

042924081

ABSTRACT:
A mass, and method of preparing such mass, and using the same for adsorbing metal ions from a physiological solution by means of living cells suspended in the solution. The cells suspended in the solution at a density of up to 20% by weight are exposed to an electric field of such intensity and over such a period of time that the membrane of the cells will be so changed that substances present in the interior of the cells and having a diameter within the range of from 2 to 50 A, such as potassium and proteins, pass from the interior of the cells into the physiological solution. The electric force or intensity is limited in such a way that the changes in the membrane of the cells can be healed by regeneration of the cells. The mass is introduced into the aqueous solution, such as sea water, lake water or waste water, and left therein until the mass as adsorbed therefrom the metal at a desired concentration, whereupon the mass is separated from the aqueous solution.

REFERENCES:
patent: 3095359 (1963-06-01), Heller
patent: 4081340 (1978-03-01), Zimmermann et al.
Pilwat, et al., "Dielectric Breakdown Measurements of Human and Bovine Errecyte Membranes Using Benzyl Alcohol as a Probe Molecule", Biochim. Biophys. Acta, vol. 406, (1975), pp. 424-432.
Belton, et al., "Effects of Pharmacalagical agents on the Electrical Responses of Cells of Nitella Flexilis", Chem. Abstracts, vol. 76, No. 3, p. 68, (1972), Abs. No. 10704n.

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