Chemistry: analytical and immunological testing – Optical result
Reexamination Certificate
2008-08-27
2009-11-10
Shibuya, Mark L (Department: 1641)
Chemistry: analytical and immunological testing
Optical result
C436S056000, C436S063000, C436S166000, C436S151000, C436S169000, C436S170000, C436S172000, C436S517000, C436S518000, C436S524000, C436S528000, C436S531000, C436S534000, C436S535000, C436S800000, C436S805000, C436S823000, C435S029000, C435S034000, C356S317000, C356S318000, C356S417000, C250S458100, C250S459100, C250S461200
Reexamination Certificate
active
07615376
ABSTRACT:
In a process for the quantitative optical analysis of fluorescently labelled biological cells5, a cell layer on a transparent support at the bottom2of a reaction vessel1is in contact with a solution3containing the fluorescent dye4. The sensitivity of analytical detection can be considerably improved if to the fluorescent dye4already present in addition a masking dye9, which absorbs the excitation light6for the fluorescent dye4and/or its emission light7, is added to the solution3and/or if a separating layer10permeable to the solution and absorbing and/or reflecting the excitation light6or the emission light7is applied to the cell layer at the bottom2. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. The separating layer10must in this case be composed such that it has a high power of reflection for the luminescent light11. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components. In this case, a receptor layer12at the bottom2of a reaction vessel1is in contact with a solution (supernatant3) in which a fluorescent or luminescent ligand13is dissolved. The sensitivity and accuracy of the analytical detection can be considerably improved here if a masking dye9which absorbs the excitation light6for the fluorescent dye and/or its emission light or (in the case of luminescent ligands) the luminescent light is added to the supernatant3. Instead of the masking dye in the solution3or optionally as an additional measure, a separating layer10permeable to the solution3and absorbing and/or reflecting the excitation light6and/or the emission light or the luminescent light can be applied to the cell or receptor layer12at the bottom2.
REFERENCES:
patent: 4476231 (1984-10-01), Deindoerfer et al.
patent: 4613567 (1986-09-01), Yasoshima et al.
patent: 4639421 (1987-01-01), Sage, Jr.
patent: 4665024 (1987-05-01), Mansour
patent: 4837168 (1989-06-01), De Jaeger et al.
patent: 4891324 (1990-01-01), Pease et al.
patent: 5082768 (1992-01-01), Burd et al.
patent: 5164301 (1992-11-01), Thompson et al.
patent: 5489537 (1996-02-01), Van Aken
patent: 5556764 (1996-09-01), Sizto et al.
patent: 5573909 (1996-11-01), Singer et al.
patent: 5582982 (1996-12-01), Cubbage et al.
patent: 5821066 (1998-10-01), Pyle et al.
patent: 5830766 (1998-11-01), Attridge et al.
patent: 5891733 (1999-04-01), Inoue
patent: 5928907 (1999-07-01), Woudenberg et al.
patent: 6200762 (2001-03-01), Zlokamik et al.
patent: 6214563 (2001-04-01), Negulescu et al.
patent: 6221612 (2001-04-01), Knapp et al.
patent: 6420183 (2002-07-01), Krahn et al.
patent: 7138280 (2006-11-01), Krahn et al.
patent: 2001/0006820 (2001-07-01), Knapp et al.
patent: 6226332 (1983-04-01), None
patent: 3213183 (1983-10-01), None
patent: 0091046 (1983-10-01), None
patent: 0558827 (1993-09-01), None
patent: 0708334 (1996-04-01), None
patent: 2103362 (1983-02-01), None
patent: 9302208 (1993-02-01), None
patent: 9323492 (1993-11-01), None
patent: 9402642 (1994-02-01), None
patent: 9417388 (1994-08-01), None
patent: 9745739 (1997-12-01), None
patent: 9804273 (1998-02-01), None
Hansson, Y., et al; “A rapid method for detection of cellular proliferation using carboxyfluorescein”; Journal of Immunological Methods, 100 (1987); pp. 2561-2567.
Wan, et al; J. Immunol. Methods, 162, pp. 1-7 (1993).
Database WPI section Ch, Week 198948; Derwent Publications Ltd., London, GB; Class B04, AN 1989-353220; XP002122217 & JPO 1265034 A (Taiyo Kagaku KK) Oct. 23, 1989 Abstract.
Passive protection of neonatal calves against bovine coronavirus-induced diarrhea by Administration of Egg Yolk or Colostrum Antibody Powder; Veterinary Microbiology 58 (1997) 105-111.
Nakajima;“New Primer to Immunology”; Nanzandoh, Second Edition, pp. 178, L4-179, L6. (translation).
Laskey et al; “Calcium entry-dependent oscillations of cytoplasmic calcium concentration in cultured endothelial cell monolayers”; Proc. Natl. Acad. Sci, USA, vol. 89, pp. 1690-1694, Mar. 1992.
Schroeder et al; “FLIPR: A new instrument for accurate, high throughput optical screening”: Journal of Biomolecular Screening, vol. 1, No. 2, 1996, pp. 75-80.
Epps, et al; “Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells”; Chemistry and Physics of Lipids, 69, (1994), pp. 137-150.
Nakajima; “New Primer to Immunology”; Nanzandoh, Second Edition, pp. 178, L4-179, L6. (translation), Nov. 24, 1999.
Bechem Martin
Krahn Thomas
Paffhausen Wolfgang
Schade Andreas
Schmidt Delf
Bayer Schering Pharma Aktiengesellschaft
Do Pensee T
Norris McLaughlin & Marcus PA
Shibuya Mark L
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