Marker, method and an assay for detection and monitoring of late

Details Marker, method and an assay for detection and monitoring of late Marker, method and an assay for detection and monitoring of late

1996-11-19

2000-02-15

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 912, 435 9121, 435 9151, 536 2432, 536 2433, 536 241, 536 231, C12Q 168, C07H 2104, C12P 1934

Patent

active

060251247

ABSTRACT:
A TAR marker, method for detection and monitoring of HIV latency and activation and an assay for detection of the marker. The assay sensitively detects HIV transcription and monitors HIV transcriptional activity by detecting the presence of TAR fragments and full length transcripts, quantifying both and determining the ratio of short to long transcripts. A low ratio correlates with a latent-type transcriptional activity of HIV whereas the appearance of long transcripts signifies increased efficiency of transcriptional activity of HIV and the transition from latency to activation. The size difference between the TAR fragments appearing predominantly in latency and the full length transcripts appearing predominantly during the HIV activation is detected by RT-PCR assay that utilizes novel primers and probes. The obtained ratio is a sensitive tool in detection of HIV infection, the analysis of load of latent and active virus and monitoring the transition from the latent to active state of HIV replication.

REFERENCES:
Adams et al, Proceedings National Academy of Sciences, USA (Apr. 1994) 91:3862-3866.
Buck et al, Science (1990) 248:208-212.

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