Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1984-03-21
1988-06-28
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
4351721, 4351723, 435874, 935 84, C12Q 104, C12N 1500, C12N 120, C12R 138
Patent
active
047538767
ABSTRACT:
This invention discloses the use of marker genes which do not involve antibiotics for environmental tracking of microorganisms. Such marker genes include chromogenic marker genes, and marker genes that allow a cell to proliferate on media containing a sole nutrient source which cannot be utilized by untransformed cells. Genetic transformation using such marker genes is used to create cells with two or more phenotypic traits that do not coexist in natural, untransformed cells. As one example, pseudonomad cells have been transformed with beta-galactosidase and lactose permease genes, to create cells which are (1) fluorescent, (2) able to hydrolyze X-gal or ONPG, and (3) capable of proliferation on lactose as a sole carbon source. Such cells are useful as soil inoculants, and their descendants can be tracked by using these characteristics. The marker genes may be placed under the control of inducible promoters.
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Drahos David J.
Hemming Bruce C.
Herald William J.
Hoerner, Jr. Dennis R.
Marantz Sidney
Monsanto Company
Williams, Jr. James W.
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