Marker for revealing contaminants and application method for per

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 32, 435 29, 435 34, 435 4, 435 36, 435 12, 435882, 435883, 435884, 435260, C12Q 122, C12Q 118, C12Q 100, C12N 100

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active

057597995

ABSTRACT:
An incubation limit marker for revealing the growth of contaminants present in a sample includes at least one strain or category of classically contaminant bacteria in dehydrated form which, once regenerated, will be present at a maximum concentration of 10.sup.3 CFU/ml. The incubation limit marker also includes a medium used for the dehydration of the contaminant bacterium or bacteria which includes a substrate capable of being degraded by the bacterium or bacteria. The incubation limit marker further includes an indicator of the growth of the bacterium or bacteria, for example, constituted by a colored indicator of changes in pH. A process for the "in vitro" detection of the susceptibility of pathogenic bacteria to various antibiotics present in MIC (i.e., Minimal Inhibitory Concentration) in culture or antibiogram media is carried out directly from the sample of the infected medium, in the presence of the above marker, used as a signal which limits the reading time of the antibiogram.

REFERENCES:
patent: 5071749 (1991-12-01), Kondo et al.
"ECCLS Standard for Antimicrobial Susceptibility Testing Diffusion Methods" ECCLS, 1985, 5:4.
28th report in the series of technical reports N, 610, WHO Geneva, 1977, 106-138 by the WHO Committee of Experts in Biological Standardization.
"New Approaches to the Study of Susceptibility of Bacteria to Antibiotics" (English language translation of Nouvelles approaches de l'etude de sensibilite des bacteries aux antibiotiques), by J.P. Flandrois, G. Carret, in the Revue Francaise du Laboratiore, vol. 151, 1986.
Libehal et al, "Preliminary Observations on the in Vitro sensitivity of Mycoplasma and Acholeplasma strains of bovine onigen," Rev. Microbiol. 1991, 22, 55-59.

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