Marker for early detection of human hydatidiform moles and chori

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 71, 435810, 536 26, 536 27, 536 28, 536 29, 530326, 530328, 5303879, 5303882, 53038885, 5303891, 5303897, 935 77, 935 78, 436501, 436 94, C12Q 168, G01N 3353, A61K 3702, C07H 1512

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051418495

ABSTRACT:
The present invention relates to a DNA segment comprising linked pregnancy-specific beta.sub.1 -glycoprotein (PS.beta.G) genes encoding PSGGA protein and PSGGB protein. The invention further relates to a probe (DNA, RNA or peptide probe) specific for PSGGB mRNA or protein expressed in human hydatidiform molar trophoblastic tissue and to a bioassay for the detection of gestational trophoblastic diseases. The PSGGB-specific probes of the present invention hybridized most strongly with RNA from molar trophoblastic tissue, suggesting that the PSGGB-like species may be the gene preferentially expressed in gestational trophoblastic diseases.

REFERENCES:
Leslie et al., Linkage of two human pregnancy-specific .beta..sub.1 -glycoprotein genes: One is associated with hydatidiform mole, Proc. Natl. Acac. Sci., vol. 87, pp. 5822-5826, Aug. 1990.
Zimmermann et al., cDNA Cloning Demonstrates the Expression of Pregnancy-Specific . . . , Biochemical and Biophysical Research communications, vol. 163, No. 3, Sep. 1989, pp. 1197-1209.
Watanabe et al.., J. Biol. Chem. 263(4): 2049-2054 (Feb. 5, 1988).

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