Marker for cerebral apoplexy

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 26, 435 4, 435 21, 435 15, 435 14, 435183, 435194, 436 63, 436 74, 562587, 562589, C12Q 134, C12Q 132, C12Q 142, C12Q 148

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057891869

ABSTRACT:
A sample is treated with a phosphoglyceric acid mutase (PGAM) inhibitor (polythionic acid (salt) such as potassium tetrathionate) to inactivate the M-type isozyme activity and the B-type PGAM is quantified by determining PGAM isozymes by a rate assay. The B-type PGAM is a novel marker for cerebral apoplexy, and the diagnosis of cerebral apoplexy is enabled by assaying it.

REFERENCES:
Uchida et al, Japanese Jour of Clinical Chem, vol. 23, No. hosatsu2, p. 113b (1994) Month not available.
Batke, et al, Archives of Biochem and Biophysics, vol. 264, No. 2, Aug. 1, 1988, pp. 510-518.
Granstrom et al, "Inactivation of Type MM Phosphoglycerate Mutase by Sulfhydryl Group Reagents During Facial Embryogenesis", Acta Odontologica Scandinavica, 46:5 pp. 273-279, 1988.
Carreras et al, "Phylogeny and Ontogeny of the Phosphoglycerate Mutases-III. Inactivation of Rabbit Muscle Phosphoglycerate Mutase (Type M Isozyme) by the Sulfhydryl Group Reagents", Comparative Biochemistry and Physiology, 71:1, pp. 57-63, 1982.
Biological Physical Chemistry, Japanese Journal of Electrophoresis, 1995, vol. 39 No. 2, pp. 1-6.
Isozymes III Developmental Biology, Clement L. Markert, Academic Press 1975, pp. 1006 thru 1018.
619th Meeting Cambridge, S. Yates et al, Plasma phosphoglycerate mutase muscle (M) isoenzyme is stikingly raised in Duchenne muscular dystrophy vol. 14 pp. 1165 thru 1166.

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