Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1994-03-03
1996-11-19
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 912, 435 9121, 435 9151, 536 2432, 536 2433, 536 241, 536 231, 935 8, 935 17, 935 18, 935 78, C07H 2104, C12Q 170, C12Q 168, C12P 1934
Patent
active
055761763
ABSTRACT:
A marker and method for detection and monitoring of HIV latency and activation and an assay for detection of the marker. The assay sensitively detects HIV transcription and monitors HIV transcriptional activity by detecting the presence of short and long transcripts, quantifying both and determining the ratio of short to long transcripts. Short transcripts are abundant and a low ratio correlates with a latent-type transcriptional activity of HIV whereas the appearance of long transcripts signifies increased efficiency of transcriptional activity of HIV and the transition from latency to activation. The size difference between the TAR fragments appearing predominantly in latency and the full length transcripts appearing predominantly during the HIV activation is detected by RT-PCR assay that utilizes novel primers and probes. The results are expressed as a ratio of short to long transcripts. The obtained ratio is a sensitive tool in detection of HIV infection, the analysis of load of latent and active virus and monitoring the transition from the latent to active state of HIV replication.
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Adams Melanie
Busch Michael P.
Peterlin Boris M.
Romeo Joseph
Jones W. Gary
Myers Carla
The Regents of the University of California
Verny Hana
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