Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Nonplant protein is expressed from the polynucleotide
Reexamination Certificate
2005-03-29
2005-03-29
Nelson, Amy J. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Nonplant protein is expressed from the polynucleotide
C435S468000
Reexamination Certificate
active
06872871
ABSTRACT:
Protein Fragment Complementation Assays (PCA) are done in plant material using enzyme fragment constructs, for example, dihydrofolate reductase (DHFR) fragment constructs. Plant material is transformed with at least two different constructs that form different products capable of interacting to reconstitute enzymatic activity in the plant material. Detection of the activity can be done using a substrate for the enzyme in the culture medium which, when reacted with the enzyme, is converted to a detectable product. One embodiment uses a substrate that can be enzymatically converted to a detectable fluorescent product. An inducer such as rapamycin or salicylic acid can be added to the culture medium to increase the level of detectable product.
REFERENCES:
patent: 5610042 (1997-03-01), Chang et al.
patent: 6392119 (2002-05-01), Gutterson et al.
Florack et al. Expression of giant silkmoth cecropin B genes in tobacco. Transgenic Research, 1995, vol. 4, pp. 132-141.*
Michnick et al. Detection of protein-protein interactions by protein fragment complementation strategies. 2000, Methods in Enzymology, vol. 328, pp. 208-230.*
Pelletier et al. Oligomerization domain-dircetd reassembly of active dihydrofolate reductase from rationally designed fragments Oct. 1998, Proc. Natl. Acad. Sci. USA, vol. 95, pp. 12141-12146.*
Remy et al. Erythropoietin receptor activation by a ligand-induced comformation change. Feb. 1999, Science, vol. 283, pp. 990-993.
Brisson Normand
Michnick Stephen William Watson
Angres Isaac A.
Collins Cynthia
Nelson Amy J.
Odyssey Thera Inc.
LandOfFree
Mapping molecular interactions in plants with protein... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mapping molecular interactions in plants with protein..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mapping molecular interactions in plants with protein... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3407944