Manufacturing process for the production of purified transferrin

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530412, 530416, 530380, 530395, 424 85, 514 8, 514724, A61K 3800, C07K 500, C07K 700, C07K 1600

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active

057445869

ABSTRACT:
Method for obtaining highly purified transferrin from a partially purified plasma fraction containing transferrin in which the starting fraction is concentrated. Its ionic strength is reduced and then transferrin is adsorbed onto a chromatographic column. Following elution, the transferrin can be further processed through to packaging in final containers. The final purified transferrin product is sterile, at least 95% pure and is substantially free of enveloped and non-enveloped viruses.

REFERENCES:
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patent: 4841026 (1989-06-01), Van Beveren et al.
patent: 5041537 (1991-08-01), Bethke et al.
Harris, et al., Protein Purification Methods A Practical Approach, pp. 5-9, 126-148, 1989.
Soliman, et al., A Medium Scale Method for the Purification of Human Serum Transferrin and IgG Globulin by Chromatographic Technique, U.A.R. J. Chem., vol. 14, No. 2, pp. 177-183, 1971.

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