Manufacture of liposomes and lipid-protein complexes by ethanoli

Details Manufacture of liposomes and lipid-protein complexes by ethanoli Manufacture of liposomes and lipid-protein complexes by ethanoli

1997-03-04

2000-09-19

Woodward, Michael P.

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

424 121, 514 2, 514 12, C07K 14785, A61K 3516, A61K 9121, A61K 900

Patent

active

061207950

ABSTRACT:
A method for preparing liposomal suspensions which comprises preparing a solution of a poorly soluble protein or other poorly soluble compound and an encapsulating amount of a suitable lipid in an alcoholic solvent, injecting the resulting solution into an aqueous solution of appropriate ionic strength, and removing the alcoholic solvent by thin-film evaporation at the transition temperature of the liposomal suspension. This process is suitable for continuous production on an industrial manufacturing scale. It is of particular application in the preparation of KL4 pulmonary surfactant composition, wherein the thin film evaporation is conducted within the transition temperature range of the surfactant (approximately 35-50.degree. C.), thereby enhancing the KL4 peptide insertion into the lipid bilayer and leading to a product with reduced viscosity and enhanced surface tension lowering properties.

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Szoka et al. Ann. Rev. Biophys. Bioeng. 9:467-508, Jan. 1980.
Biochimica Et Biophysica Acta, 298(1973) pp. 1015-1019 Elsevier Scientific Publishing Company, Amsterdam. Single Bilayer Liposomes Prepared Without Sonication.

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