Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...
Patent
2000-01-18
2000-11-14
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing heterocyclic carbon compound having only o, n, s,...
435137, 435195, 435196, 435197, C12P 1704, C12N 918
Patent
active
061468606
ABSTRACT:
A process for producing L-ascorbic acid (vitamin C) from 2-keto-L-gulonic acid or D-erythorbic acid from 2-keto-D-gluconic acid by contacting 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, respectively, in solution with a lactonase, particularly one belonging to the enzyme class EC 3.1.1.x, according to the classification of Enzyme Nomenclature. The solvent for this reaction can be water, an aqueous alcohol, a non-alcoholic organic solvent or a mixture of an aqueous alcohol and a non-alcoholic organic solvent. The contacting is generally performed in a temperature range of 0.degree. C. to 120.degree. C. and a pH range of 1.5 to 12. In each case the starting material can be in the form of the free acid, the sodium salt, or the calcium salt. The so-produced vitamin C has very well known uses, and the alternatively produced D-erythorbic acid is useful as an antioxidant for food additives.
REFERENCES:
patent: 4259443 (1981-03-01), Danehy
patent: 5817490 (1998-10-01), Hubbs
patent: 5859262 (1999-01-01), Meir-Eyal et al.
Ogawa, et al., "Microbial enzymes: new industrial applications from traditional screening methods," TIBTECH, vol. 17, pp. 13-20 (1999).
Kobayashi, et al., "Lactone-ring-cleaving enzyme: Genetic analysis, novel RNA editing, and evolutionary implications," Proc. Natl. Acad. Sci. USA, vol. 95, pp. 12787-12792 (2998).
Bublitz, et al., "The role of aldonolactonase in the conversion of L-gulonate to L-ascorbate," Biochimica et Biophysica, vol. 47, pp. 288-297 (1961).
Derwent English language abstract of De 196 04 798 A1 (document B2).
Zachariou, et al., "Glucose-Fructose Oxidoreductase, a New Enzyme Isolated from Zymomonas mobilis That is Responsible for Sorbitol Production," Journal of Bacteriology, 167(3): 863-869 (1986).
Hucho, et al., "Glucono-.delta.-Lactonase From Escherichia Coli," Biochimica et Biophysica Acta, 276:176-179 (1982).
Shimizu, et al., "Purification and Characterization of a Novel Lactonohydrolase, Catalyzing the Hydrolysis of Aldonate Lactones and Aromatic Lactones, from Fusarium oxysporum," Eur. J. Biochem., 209:383-390 (1992).
Kanagasundaram, et al., "Isolation and Characterization of the gene encoding gluconolactonase from Zymomonas mobilis," Biochimica et Biophysica Acta, 1171: (1992).
Asakura Akira
Hoshino Tatsuo
Kiyasu Tatsuya
Shinjoh Masako
Haracz Stephen M.
Lilling Herbert J.
Roche Vitamins Inc.
Waddell Mark E.
LandOfFree
Manufacture of L-ascorbic acid and D-erythorbic acid does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Manufacture of L-ascorbic acid and D-erythorbic acid, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Manufacture of L-ascorbic acid and D-erythorbic acid will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2063861