Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1996-05-02
2000-09-12
Housel, James C.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
5303873, 530402, 435 71, 435 691, C07K 100, C12P 2108, C12P 2106, G01N 3353
Patent
active
061179760
ABSTRACT:
Molecules comprise (i) a polypeptide (such as calmodulin) which has calcium-dependent binding affinity for ligand and (ii) another polypeptide, the polypeptides preferably being joined by a peptide bond and produced by recombinant expression from a gene fusion. The molecules are useful in detection, immobilization, targeting and purification, cell-labelling, and band-shift assays for determining binding of a member of a specific binding pair (sbp) for complementary sbp member. For purposes of band-shift assays, polypeptide (i) need not have calcium-dependent binding affinity for a ligand, but should have a dissociation constant for a ligand of 10 nM or less, measured at a pH of between 6 and 9 at 20.degree. C. In an alternative embodiment, a calmodulin-binding polypeptide which is, or is derived from, mastoparan is joined, as polypeptide (i) instead of a binding polypeptide, to the other polypeptide.
REFERENCES:
patent: 5498538 (1996-03-01), Kay et al.
Stofko-Hahn et al., "A single step purification for recombinant proteins," FEBS Letters, 302 (3):274-278, May 1992.
Beidler et al., "Cloning and High Level Expression of a Chemeric Antibody . . . ," Journal of Immunology, 141(11):4053-4060, Dec. 1988.
Jos A. Cox et al., "The Interaction of Calmodulin with Amphipilic Peptides," The Journal of Biological Chemistry, vol. 260, No. 4, pp. 2527-2534 (Feb. 25, 1985).
Kazuko Haga et al., "Synergistic Activation of a G Protein-coupled Receptor Kinase by G Protein .beta..gamma. Subunits and Mastoparan or Related Peptides," The Journal of Biological Chemistry, vol. 269, No. 17, pp. 12594-12599 (Apr. 29, 1994).
Renata E. Stofko-Hahn et al., "A single step purification for recombinant proteins. Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP-dependent protein kinase," FEBS Letters, vol. 302, No. 3, pp. 274-278 (May, 1992).
Zhijun Luo et al., "Identification of the MAP2- and P75-binding Domain in the Regulatory Subunit (RII.beta.) of Type II cAMP-dependent Protein Kinase," The Journal of Biological Chemistry, vol. 265, No. 35, pp. 21804-21810 (Dec. 15, 1990).
Tomio Ono et al., "Molecular Cloning Sequence and Distribution of Rat Calspermin, a High Affinity Calmodulin-binding Protein," The Journal of Biological Chemistry, vol. 264, No. 4, pp. 2081-2087 (Feb. 5, 1989).
Rasera Da Silva et al., "Hybrid myosin light chains containing a calcium-specific site from toponin C," Chemical Abstracts, vol. 116, No. 12, p. 354 (Mar. 30, 1992), abstract no. 123563g.
MacKenzie, et al., "Biofunctional fusion proteins consisting of a single-chain antibody and an engineered lanthanide-binding protein," Immunotechnology, 1:139-150, 1995.
Neri et al., "Calmodulin as a Versatile Tag for Antibody Fragments," Biotechnology, 13:373-377, Apr. 1995.
De Lalla Claudia
Neri Dario
Winter Gregory Paul
Devi S.
Housel James C.
Medical Research Council
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