Manufacture and use of fowl egg antibodies

Details Manufacture and use of fowl egg antibodies Manufacture and use of fowl egg antibodies

1982-07-16

1985-10-29

Wiseman, Thomas G.

Drug, bio-affecting and body treating compositions

Lymphokine

424 86, 424 87, 424 88, 424 89, 514 6, 260112B, 260112R, 435 7, 435810, 436547, 436539, 436817, 436823, A61K 3942, A61K 3940, A61K 3912, A61K 3900

Patent

active

045500193

ABSTRACT:
Immunological preparations are prepared by immunizing hens with an immunogen having a molecular or particle weight of at least 30,000, to a stage of hyper-immunization at which there occurs a plateau-like levelling-off of the antibody content of the serum. The immunogenicity of the immunogen can be enhanced by enlarging the immunogen particle mass. The eggs of the immunized hens are collected, the yolk is separated from the eggs, followed by separation of the lipid content of the yolk. The antibodies in the egg yolk are then rendered indispersable with the aid of a water-soluble linear filamentary non-charged polymer precipitant such as PEG and the indispersable antibodies are recovered. This precipitation of antibodies is advantageously preceded by a precipitation of caseinaceous proteins, lipid and yolk particles at lower polymer concentrations. Selected antibodies (IgY or IgG) can be concentrated by pH-controlled fractional precipitation with PEG. The immunological preparations are useful for micro-assays and in appropriate cases also for the treatment or prophylaxis of pathological conditions.

REFERENCES:
patent: 2543215 (1951-02-01), Williams et al.
patent: 4021534 (1977-05-01), Lafontaine
patent: 4165370 (1979-08-01), Coval
patent: 4357272 (1982-11-01), Polson
Heller et al., Res. Vet. Sci. V 18, pp. 117-120 1975 "The Immune Response of Hens to Multiple E. Coli Injections and Transfer of Immunoglobulins to the Egg and Hatched Chick".
Stedman J. of Comp. Pathology, V. 79, No. 4, 1969 pp. 507-516.
Yamamoto Japan J. Vet. Res., V. 23 No. 4, 1975 pp. 131-140.
Williams Methods in Immunology & Immunochemistry, Academic Press, NY, V. 1 1967, pp. 209-212, 229-237, 244-255.

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