Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2005-07-19
2005-07-19
Achutamurthy, Ponnathapura (Department: 1652)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S193000, C435S252330, C435S320100
Reexamination Certificate
active
06919440
ABSTRACT:
A one-pot glycosylation reaction is disclosed in which a mannosyl (Man) group is enzymatically transferred to an acceptor molecule. The starting glycoside is a mannosyl 1-phosphate that is enzymatically converted to its GDP derivative via UTP and a pyrophorylase. The formed GDP derivative is used in the enzyme-catalyzed glycosyl transfer. That enzyme-catalyzed glycosyl transfer to an acceptor releases GDP that is enzymatically converted to GTP for further conversion of mannosyl 1-phosphate into its GDP derivative. Also disclosed are a recombinant α1,2-mannosyltransferase that is enzymatically active, is dispersible in an aqueous reaction medium, and free of the transmembrane portion of the native enzyme, as well as DNA encoding that transferase, an expression vector containing exogenous DNA that encodes that enzyme andE. colicells containing that vector.
REFERENCES:
patent: 4569909 (1986-02-01), Seno et al.
patent: 5032519 (1991-07-01), Paulson et al.
patent: 5103008 (1992-04-01), Scudder et al.
patent: 5180674 (1993-01-01), Roth
patent: 5246840 (1993-09-01), Nilsson
patent: 5264352 (1993-11-01), Thiem et al.
patent: 5278299 (1994-01-01), Wong et al.
patent: 5324663 (1994-06-01), Lowe
patent: 6485930 (2002-11-01), Wong
Enzyme Nomenclature (1992) Academic Press, Inc., San Diego pp. 210, 221, 223, 591, 636, 648, 692 and 694.
Lahav et al. (1969) J. Biol. Chem 244, 5890-5898.
Brennan et al. (1968) Biochem. Biophys. Res. Comm.,30, 69-75.
Schutzbach (1980) J. Biol. Chem. 255, 4170-4175.
Jensen et al. (1981) J. Biol. Cehm. 256, 12899-12904.
Sharma et al. (1982) Eur. J. Biochem. 126, 319-325.
Romero et al. (1989) J. Biol. Chem. 264, 1946-1950.
Wang et al., J. Org. Chem., 58:3985-3990 (1993).
Wong et al., Pure & Appl. Chem. 65(4): 803-809 (1993).
Heidlas et al., Acc. Chem. Res., 25:307-314 (1992).
Wong et al., Pure & Appl. Chem., 64:1197-1202 (1992).
David et al., Adv. Carbohydr. Chem. Biochem., 49:175 (1991).
Wong et al., J. Org. Chem., 47:5416 (1982).
Hausler et al., Glycobiology, 2: 77-84 (1992).
Lewis et al., J. Biol. Chem., 266:8255-8251 (1991).
Aoki et al. 1990, The EMBO J. 9(10): 3171-3178.
David, S., et al., Advances in Carbohydrate Chemistry and Biochemistry, vol. 49, “Enzymic methods in preparative carbohydrate chemistry”, pp. 175-237. 1991.
Stryer, L., Biochemistry, Third Edition, W.H. Freeman and Co., New York, pp. 343-344, 1988.
Achutamurthy Ponnathapura
Moore William W.
The Scripps Research Institute
Welsh & Katz, LTD
LandOfFree
Mannosyl transfer with regeneration of GDP-mannose does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mannosyl transfer with regeneration of GDP-mannose, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mannosyl transfer with regeneration of GDP-mannose will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3384092