Mannose-1-phosphate transferase gene from yeast and its use for

Details Mannose-1-phosphate transferase gene from yeast and its use for Mannose-1-phosphate transferase gene from yeast and its use for

1996-11-14

1998-12-29

Lau, Kawai

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435193, 4353201, 536 232, C12P 1904, C12P 910, C12P 1563, C07H 2104

Patent

active

058540318

ABSTRACT:
The present invention relates to a mannose-1-phosphate transferase gene from yeast (Saccharomyces cerevisiae), a process for producing a mannose-1-phosphate-containing acidic sugar chain which comprises culturing yeast cells transformed with a plasmid DNA containing said gene, obtaining mannose-1-phosphate transferase from the culture, and allowing the enzyme to act on a neutral core sugar chain in vivo or in vitro, and a process for producing a phosphate-containing acidic sugar chain which comprises removal of a mannose moiety by acid-treatment of said mannose-1-phosphate-containing acidic sugar chain. According to the present invention, an acidic sugar chain having mannose-1-phosphate added to the same neutral core sugar chain as a high mannose type sugar chain produced by mammalian (e.g. human) cells can be produced in large amounts with high purity by genetic engineering means using yeast. Further, an acidic sugar chain identical with a useful phosphate-containing core sugar chain derived from human, which can function as a labeling marker for transport of glycoproteins into lysosomes in mammalian (e.g. human) cells, can be produced in large amounts with high purity.

REFERENCES:
patent: 3713979 (1973-01-01), Slodki et al.
patent: 5135854 (1992-08-01), MacKay et al.
Winnett, E. et al. EMBL Database, Accession No. S61089, available Oct., 1995.
Wang et al. (1995) "Molecular cloning of MNN6 gene involved in mannosylphosphate addition in yeast mannan" Nippon Bunshi Seibutsu Gakkai Nenkai Puroguramu, Koen Yoshishu, vol. 18, p. 390 Abstract 1P-525.
Alberts, b. et al. Molecular Biology of the Cell, Second Edition. New York, Garland Publishing, Inc., 1989, pp. 258-262, 265, 266.
E. M. Karson et al., "Biosynthesis of Yeast Mannan," The Journal of Biological Chemistry, vol. 1253, No. 18, Issue of Sep. 25, 1978, pp. 6484-6492, Printed in U.S.A.

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