Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1985-07-02
1987-08-18
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 70, 435 71, 435 91, 4351721, 4351723, 435235, 435239, 435320, 4352402, 536 27, 424 89, 424 93, C12N 1500, C12N 500, C12N 502, C12N 700
Patent
active
046877371
ABSTRACT:
A method of suppressing a nonsense codon in a gene in a mammalian cell by preparing an oligonucleotide primer having a mismatched anticodon region corresponding to the nonsense codon; preparing a DNA template for production of a tRNA molecule enabling the insertion of an amino acid when the nonsense codon is translated; forming a suppressor gene from said template and primer by site specific mutagenesis; and transforming the suppressor gene into the cell.
REFERENCES:
Muller et al., "Nucleotide Sequence of Genes Coding for tRNA.sup.Phe and tRNA.sup.Tyr from a Repeating Unit of X. Laevis DNA", Cell 19: 345, (1980).
Wallace et al., "Directed Deletion of a Yeast Transfer RNA Intervening Sequence", Science 209: 1396, (1980).
Hamer et al., "Expression of the Shromosomal Mouse Beta.sup.mag -Globin Gene Cloned in SV40", Nature 281: 35, (1979).
Hamer, "SV40 Carrying an Escherichia Coli Suppressor Gene", in Recombinant Molecules: Impact on Science and Society, R. F. Beers, Jr. et al., (ed.), Raven Press, New York, 1977, pp. 317-335.
Hamer et al.: Chem. Abstr. 88:2962f, (1978), of Miles Int. Symp. Ser. 10, 317, (1977).
Laski et al.: Proc. Natl. Acad. Sci. U.S.A. 79, 5813, (1982).
Laski et al.: Nucleic Acids Res. 10, 4609, (1982).
Temple et al.: Nature 296, 537, (1982).
Hudziak et al.: Cell 31, 137, (1982).
Capecchi Mario R.
Laski Frank A.
RajBhandary Uttam L.
Sharp Phillip A.
Engellenner Thomas J.
Martinell James
Maslow James E.
Massachusetts Institute of Technology
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