Mammalian ribonuclease inhibitors and use thereof

Details Mammalian ribonuclease inhibitors and use thereof Mammalian ribonuclease inhibitors and use thereof

1997-08-13

1999-08-03

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 235, 4353201, 435325, 4352523, 43525233, 435419, C12P 2106, C07H 120, C12N 500, C12N 510

Patent

active

059324406

ABSTRACT:
The invention relates to the cloning of a gene encoding rat liver RI, and its expression in a cellular host. In addition, the invention relates to the successful cloning of a gene encoding porcine liver RI, and its expression in a cellular host. The invention also relates to the cloning and expression of human liver RI, and the cloning and expression of chimeric mammalian RIs, particularly chimeric porcine/human liver RIs, which may be thermostable. The invention also relates to methods and kits for use in producing nucleic acid molecules and polypeptides using the RIs of the invention, and to nucleic acid molecules and polypeptides produced using these methods and kits.

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