Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-10-19
1998-11-24
Elliott, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435325, 530326, 530328, 530350, 536 232, C12P 2100, C12N 1563, C07H 2104, C07K 1400
Patent
active
058405291
ABSTRACT:
This invention relates to a novel and seventh member of the subtilisin-kexin family isolated from rat, which has been named rPC7. The rat spleen cDNA has been totally sequenced. A shorter DNA sequence has been obtained for human, which corresponds to a portion of the catalytic region of a human pro-hormone convertase corresponding to the rat pro-hormone convertase. PC7 clearly distinguishes from the other mammalian members of the subtilisin-kexin family. Its tissue distribution is ubiquitous, but its presence is particularly remarkable in lymphoid tissues. It is present in LoVo cells that are able to cleave the HIV gp160 protein into active gp120/gp41 proteins and that are deficient in other effective pro-hormone convertases known up to date. Therefore, it is proposed that PC7 is a good candidate as a maturation enzyme responsible for the conversion of HIV gp160 protein in target CD.sup.+4 cells.
REFERENCES:
Decroly, E., et al., (1994). Journal of Biological Chemistry. 269(16):12240-12247.
Gillespie, M. T., et al. (1995). J. Cell. Biochem. Suppl. 19B, p. 243, Abstract No. B7--102.
Johnson, R. C., et al. (1994) Endocrinology. 135:1178-1185.
Ohnishi, Y., et al. (1994). Journal of Virology. 68(6):4075-4079.
Seidah, N. G., et al. (1994). Biochimie. 76:197-209.
Seizen, R. J., et al. (1994). Eur. J. Biochem. 222:255-266.
Takahashi, S., et al. (1993). Biochem. Biophys. Res. Commun. 195(2):1019-1026.
Tsuji, A. S. (1994). Biochem. Biophys. Res. Commun. 202:1452-1459.
Chretien Michel
Day Robert
Seidah Nabil G.
Clinical Research Institute of Montreal
Elliott George C.
Schwartzman Robert
LandOfFree
Mammalian pro-hormone convertase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mammalian pro-hormone convertase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mammalian pro-hormone convertase will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1700726