Mammalian pancreatic cholesterol esterase

Details Mammalian pancreatic cholesterol esterase Mammalian pancreatic cholesterol esterase

1989-11-13

1992-12-22

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525233, 4352523, 4353201, 435198, 536 27, 935 9, 935 29, 935 32, 935 73, 935 70, C12N 1555, C12N 1570, C12N 1585

Patent

active

051734087

ABSTRACT:
The invention provides methods for the purification to homogeneity of pancreatic cholesterol esterase in useful quanities from a variety of mammalian species. The gene for a mammalian pancreatic cholesterol esterase has been cloned and sequenced, and is useful for expressing cholesterol esterase in a transformed eukaryotic or prokaryotic cell culture. Thus, methods according to the invention enable the production of large quantities of pancreatic cholesterol esterase for the screening of inhibitors, the production of antibodies, and for commercial purposes related to the alteration of cholesterol/cholesterol ester composition of materials containing free or esterified cholesterol.

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