Mammalian methadone-specific opioid receptor gene and uses

Details Mammalian methadone-specific opioid receptor gene and uses Mammalian methadone-specific opioid receptor gene and uses

1993-11-08

1997-08-19

Walsh, Stephen G.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

4352523, 43525411, 4353201, 435 691, 435365, 435364, 435367, 435361, 435356, 536 235, 536 243, C12N 1512, C12N 510

Patent

active

056587830

ABSTRACT:
The present invention relates to a novel mammalian methadone-specific opioid receptor protein and genes that encode a such protein. The invention is directed toward the isolation, characterization and pharmacological use of mammalian methadone-specific opioid receptor proteins. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to the rat homologue or the mammalian methadone-specific opioid receptor gene. Also provided are recombinant expression constructs capable of expressing the mammalian methadone-specific opioid receptor genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the mammalian methadone-specific opioid receptor proteins encoded therein. The invention also provides methods for screening compounds in vitro that are capable of binding to the mammalian methadone-specific opioid receptor proteins of the invention, and further characterizing the binding properties of such compounds in comparison with known opioid receptor agonists and antagonists.

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