Mammalian methadone-specific opioid receptor and uses in binding

Details Mammalian methadone-specific opioid receptor and uses in binding Mammalian methadone-specific opioid receptor and uses in binding

1997-08-15

1998-10-13

Teng, Sally

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 721, 435 691, 4352523, 43525411, 4353201, 435325, 530350, C12Q 102, C07K 14705

Patent

active

058210679

ABSTRACT:
The present invention relates to a novel mammalian met one-specific opioid receptor protein and genes that encode a such protein. The invention is directed toward the isolation, characterization and pharmacological use of mammalian methadone-specific opioid receptor proteins. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to the rat homologue or the mammalian methadone-specific opioid receptor gene. Also provided are recombinant expression constructs capable of expressing the mammalian methadone-specific opioid receptor genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the mammalian methadone-specific opioid receptor proteins encoded therein. The invention also provides methods for screening compounds in vitro that are capable of binding to the mammalian methadone-specific opioid receptor proteins of the invention, and further characterizing the binding properties of such compounds in comparison with known opioid receptor agonists and antagonists.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Nilsen et al.
Gluzman et al., (1981) Cell, 23:175-182.
Walters, (1993) "Computer-Assisted Modeling of Drugs." Pharmaceutical Biotechnology, pp. 165-174.
Thomas & Capecchi, (1987) Cell, 51: 503-512.
Bertling, (1987) Bioscience Reports, 7: 107-112.
Smithies et al., (1985) Nature, 317: 230-234.
Kieffer et al., (1992) Proc. Natl. Acad. Sci., 89: 12048-12052.
Evans et al., (1992) Science, 258: 1952-1955.
Chirgwin et al., (1979) Biochemistry, 18: 5294-5299.
Saiki et al., (1988) Science, 239: 487-491.
Sanger et al., (1977) Proc. Natl. Acad. Sci., 74: 5463-5467.
Eisenberg et al., (1984) J. Molec. Biol., 179: 125-142.
Chen & Okayama, (1987) Molec. Cell. Biol., 7: 2745-2752.
Bunzow et al., (1988) Nature, 336: 783-787.
Arizza et al., (1988) Neuron, 1: 887-900.
Blakely et al., (1991) Anal. Biochem., 194: 302-308.
Felgner et al., (1987) Proc. Natl. Acad. Sci. 84: 7413-7417.
Arizza et al., (1992) J. Neurosci., 12: 4045-4055.
Smith & Johnson, (1988) Gene, 67: 31-40.
Chen et al., (1993) "Molecular Cloning and Functional Expression of a .nu.-Opioid Receptor from Rat Brain." Molec. Pharmacol. 44: 8-12--P.
Yasuda et al., (1993) "Cloning and functional comparison of .kappa. and .delta. opioid receptors from mouse brain." Proc. Natl. Acad. Sci., 90: 6736-6740.
Bzdega et al., (1993) "Regional expression and chromosomal location of the .delta. opiate receptor gene.", Proc. Natl. Acad. Sci., 90:9305-9309.
Brownstein et al., (1993) "A brief history of opiates, opioid peptides amid opioid receptors." Proc. Natl. Acad. Sci., 90: 5391-5393.
DiChara & North, (1992) "Neurobiology of opitate abuse." Trends in Phamacol. Sci., 13:185-193.
Maneckji and Minna, (1992) "Nonconventional opioid binding sites mediate growth inhibijtory effects of methadone on human lung cancer cells." Proc. Natl. Acad. Sci., 89: 1169-1173.
McKnight & Rees, (1991) "Opioid Receptors and their Ligands." Neurotransmissions, 7: 1-6.
Goldstein, (1987) "Binding selectivity profiles for ligands of multipe receptor types: focus on opiod receptors." Trends in Pharmacol. Sci., 8: 456-459.
Kristensen et al., (1995) Life Sciences, vol. 56, p. 56.
Fukuda et al., (1993) FEBS Letters, vol. 327, p. 311.
Wang et al., (1993) PNAS, vol. 90, p. 10230.
Reeck et al., (1987) Cell, vol. 50, p. 667.
Lewin, (1987) Science, vol. 23.

Affiliated with

Also associated with

Rating

Mammalian methadone-specific opioid receptor and uses in binding does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mammalian methadone-specific opioid receptor and uses in binding, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mammalian methadone-specific opioid receptor and uses in binding will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-312279