Mammalian expression vectors

Details Mammalian expression vectors Mammalian expression vectors

1995-03-28

1997-05-06

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9141, 4351723, 4353201, 435325, 435358, 435365, C12Q 168

Patent

active

056270330

ABSTRACT:
A vector system that allows the rapid and effective screening of recombinant constructs. The vector system includes a marker protein useful for identifying transfected cell lines, wherein the promoter used to express the marker protein has be substantially weakened in comparison to its corresponding wild type form.

REFERENCES:
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patent: 5266683 (1993-11-01), Oppermann et al.
R.J. Kaufman, "Selection and Coamplification of Heterologous Genes in Mammalian Cells", in Methods in Enzymology, 185:537-566 (1990).
R.J. Kaufman, "Vectors Used for Expression in Mammalian Cells" in Methods in Enzymology, 185:487-511 (1990).
Kim et al., (Abstract) "Effects of Multiple Mutations at the Conserved TATA Sequence of Bacteriophage SP6 Promoter on Transcription Activity", Biochem. and Mol. Biol. Int. 31(1):153-9 (1993).
Mulligan and Berg (Proc. Natl. Acad. Sci., 78:2072-2076 (1981)).
Pauly, et al., "The Initiation of Accuracy of the SV40 Early Transcription is Determined by the Functional Domains of Two TATA elements", Nucleic Acids Res., 20(5):975-982 (1992).
"Expression of Proteins" Chapter 16, Expression of Cloned Genes in Cultured Mammalian Cells, in Molecular Cloning: A Laboratory Manual, J. Sambrook ed, 2nd ed., pp. 16.3-16.31 (1989).
Japanese Publ. No. 04-179485 (Abstract) DIALOG File 347 (JAPIO).
WPI Acc. No. 92-263665/32 (Abstract) DIALOG File 351 (Derwent WPI).
Niwa et al., "Efficient Selection for High-expression Transfectants with a Novel Eukaryotic Vector", Gene 108:193-199 (1991).

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