Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2011-04-05
2011-04-05
Ketter, James S (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S358000, C435S320100
Reexamination Certificate
active
07919270
ABSTRACT:
The present invention relates to protein expression systems and in particular to a mammalian protein expression system. Specifically, the present invention provides a rodent cell line with enhanced protein production capabilities.The invention also relates to eukaryotic cloning and expression vectors and related methods, and in particular to DNA vectors capable of high level expression of a protein of interest. The invention allows for long-term episomal maintenance of expression vectors in mammalian cells.
REFERENCES:
patent: 5374515 (1994-12-01), Parenteau et al.
patent: 5798105 (1998-08-01), Schoenmakers et al.
DeWaele et al., Eur. J. Biochem. vol. 176, pp. 287-295 (1988).
Heinzel et al., “Use of Simian Virus 40 Replication to Amplify Epstein-Barr Virus Shuttle Vectors in Human Cells”, Journal of Virology, 1988, 62(10), pp. 3738-3746.
Stary and Sarasin,“Simian virus 40 (SV40) large T antigen-dependent amplification of an Epstein-Barr virus-SV40 hybrid shuttle vector integrated into the human HeLa cell genome”, Journal of General Virology, 1992, 73, pp. 1679-1685.
Haase et al., “Improved EBV shuttle vectors”, Mutation Research, 1989, 220, pp. 125-132.
Piechaczek et al.,“A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells”, Nucleic Acids Research, 1999, 27(2), pp. 426-428.
International Search Report of PCT/AU2004/001224, (2005).
Mizuguchi H et al, “Long-term replication of Epstein-Barr virus-derived episomal vectors in the rodent cells”, Febs Letters, Elsevier, Amsterdam, NL, vol. 472, No. 2-3, Apr. 28, 2000, pp. 173-178.
Heffernan M et al, “Polyoma and Hamster papovavirus large T antigen-mediated replication of expression shuttle vectorsin Chinese hamster ovary cells” Nucleic Acids Research, Oxford University Press, Surry, GB, vol. 19, No. 1, 1991, pp. 85-92.
Andersen Dana C et al, “Recombinant protein expression for therapeutic applications”, Current Opinion in Biotechnology, vol. 13, No. 2, Apr. 2002, pp. 117-123.
Kunaparaju Rajkumar et al, Epi-CHO, an episomal expression system for recombinant protein production in CHO cells, Biotechnology and Bioengineering, vol. 91, No. 6, Sep. 2005, pp. 670-677.
Pham P L et al, “Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: Peptone additives improve cell growth and transfection efficiency”, Biotechnology and Bioengineering, Wiley & Sons, Hoboken, NJ, US, vol. 84, No. 3, Nov. 5, 2003, pp. 332-342.
Meissner P et al, “Transient gene expression: Recombinant protein production with suspension-adapted HEK293-EBNA cells”, Biotechnology and Bioengineering—Combinatorial Chemistry Wiley, New York, US, vol. 75, No. 2, Oct. 20, 2001, pp. 197-203.
Polvino-Bodnar M et al, “DNA binding activity is required for EBNA 1-dependent transcriptional activation and DNA replication”, Virology, vol. 187, No. 2, 1992, pp. 591-603.
European Search Report re: EP 04 81 6094, dated Jun. 8, 2007.
Kunaparaju Rajkumar
Sunstrom Noelle-Ann
Acyte Biotech Pty Ltd
Edwards Angell Palmer & & Dodge LLP
Hsi Jeffrey D.
Ketter James S
Spar Elizabeth
LandOfFree
Mammalian expression system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mammalian expression system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mammalian expression system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2703357