Mammalian endonuclease III, and diagnostic and therapeutic uses

Details Mammalian endonuclease III, and diagnostic and therapeutic uses Mammalian endonuclease III, and diagnostic and therapeutic uses

1997-02-27

1999-02-16

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C12N 922

Patent

active

058719920

ABSTRACT:
The present invention relates to isolated mammalian homologues of the DNA repair enzyme, E. coli endonuclease III. These mammalian enzymes have a glycosylase/AP lyase activity. Included in the present invention are fusion proteins containing the active mammalian endonuclease III. Also included are nucleic acids encoding the mammalian enzymes of the present invention and fusion proteins thereof, along with cloning, and expression vectors which contain these nucleic acids. In addition, associated diagnostic, therapeutic and pharmaceutical materials are included, along with related methods of making and using the same. The present invention also includes methods of expressing the recombinant forms of mammalian endonuclease III enzymes and fusion proteins thereof, and methods for isolating these expressed enzymes and proteins, as well as the methods for isolating the natural forms of mammalian endonuclease III enzymes.

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