Mammalian cell-based DNA libraries

Details Mammalian cell-based DNA libraries Mammalian cell-based DNA libraries

1991-12-16

1994-01-04

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352523, 4353201, 536 235, C12N 1509

Patent

active

052759423

ABSTRACT:
A recombinant plasmid useful for the production of large-insert, stably maintained, episomes in mammalian cells is disclosed, along with cells, DNA libraries, and methods of using the same. The plasmid comprises a lymphotrophic herpes virus segment (e.g., an Epstein-Barr virus segment) containing an origin of plasmid replication (oriP) and a heterologous insert segment linked to the lymphotrophic herpes virus segment. The heterologous insert segment has a length of at least 100 kilobases. In a preferred embodiment, the lymphotrophic herpes virus segment is capable of producing infectious virions in a suitable host cell, and the lymphotrophic herpes virus segment has regions deleted so that the recombinant plasmid retains the capability of producing infectious virions in the host.

REFERENCES:
patent: 4686186 (1987-08-01), Sugden
W. Hammerschmidt and B. Sugden, Cell 55, 427-433 (1988).
W. Hammerschmidt and B. Sugden, Nature 340, 393-397 (1989).
J.-M. Vos et al., Molecular Carcinogenesis 2, 237-244 (1989).
J-M. Vos and P. Hanawalt, Mutation Research 220, 205-220 (1989).

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