Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1996-10-29
1998-02-24
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351722, 4351723, 4353201, 435325, 514 44, 800 2, 536 231, 536 235, 935 11, 935 89, 935 90, 935 92, 935 93, 935 95, 935 96, 935106, 935107, C12N 1506, C12N 1512, C12N 1516, C12N 528
Patent
active
057211183
ABSTRACT:
The present invention provides a mammalian artificial chromosome (MAC), comprising a centromere and a unique cloning site, said MAC containing less than 0.1% of the DNA present in a normal haploid genome of the mammalian cell from which the centromere was obtained. The invention further provides a MAC, wherein the unique cloning site is a nucleic acid sequence encoding a selectable marker. The invention also provides methods of preparing a MAC. In addition, the invention provides methods of stably expressing a selectable marker in a cell, comprising introducing a MAC containing the selectable marker into the cell. The invention also provides a cell containing a MAC expressing an exogenous nucleic acid sequence and a transgenic mammal expressing a selectable marker.
REFERENCES:
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Chambers Jasemine C.
Schmuck Jill D.
The Regents of the University of California, San Diego
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