Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal
Patent
1998-02-17
2000-10-17
Campell, Bruce R.
Multicellular living organisms and unmodified parts thereof and
Method of making a transgenic nonhuman animal
800 24, 800 25, C12N 1500
Patent
active
061335037
ABSTRACT:
The present invention provides a mammalian artificial chromosome (MAC), comprising a centromere and a unique cloning site, said MAC containing less than 0.1% of the DNA present in a normal haploid genome of the mammalian cell from which the centromere was obtained. The invention further provides a MAC, wherein the unique cloning site is a nucleic acid sequence encoding a selectable marker. The invention also provides methods of preparing a MAC. In addition, the invention provides methods of stably expressing a selectable marker in a cell, comprising introducing a MAC containing the selectable marker into the cell. The invention also provides a cell containing a MAC expressing an exogenous nucleic acid sequence and a transgenic mammal expressing a selectable marker.
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Campell Bruce R.
The Regents of the University of California
Woitach Joseph
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