Mammalian adipocyte protein p154, nucleic acids coding therefor

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435 691, 435 701, 435 703, 435 711, 4351723, 4352402, 4353201, 536 235, 935 11, 935 27, 935 56, 935 66, C12N 1512, C12N 1563

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052682951

ABSTRACT:
A mammalian adipocyte-specific polypeptide, termed p154, is expressed in high quantities in adipogenic cell lines after cell differentiation and is abundant in the fat pads of normal and genetically obese mammals. The murine and human polypeptide, DNA and RNA molecules coding therefor, methods for its preparation, and antibodies specific for the polypeptide are also disclosed. Methods for determining the susceptibility of a subject to obesity are based on measuring the levels of the adipocyte polypeptide in a biological fluid or tissue extract or by measuring mRNA encoding the polypeptide in cells of the subject. Methods of evaluating an anti-obesity drug comprise contacting the drug with an adipocyte in vitro and measuring the amount of the adipocyte polypeptide or mRNA produced by the adipocyte. Methods of treating a subject with obesity comprise administering the above antibody or blocking the expression of the p154 gene.

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