Maltooligoside derivative, reagent for determining .alpha.-amyla

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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536 41, 536 173, 536 175, 536 177, 536 184, 536 187, 53612313, 435 96, 435 98, 435 99, C12Q 140

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active

053025148

ABSTRACT:
The present invention provides a maltooligoside derivative represented by the formula: ##STR1## wherein n denotes an integer 3-5, R represents an aromatic chromophoric group, X represents a group >CHCH.sub.2 N.sub.3 or >C.dbd.CH.sub.2, and Y represents a hydrogen atom, a substituted or unsubstituted hydrocarbon group or an alkyl- or arylsulfonyl group, a reagent for determining .alpha.-amylase activity which comprises said maltooligoside derivative as an effective ingredient, and a process for determining .alpha.-amylase activity, characterized in that said maltooligoside derivative and coupled enzymes are added to an .alpha.-amylase containing sample to conduct an enzymatic reaction and a liberated aromatic chromophoric compound is quantitatively determined. The compound of the present invention is very useful as a substrate for determining .alpha.-amylase activity, and .alpha.-amylase activity can be easily measured accurately in a short time by using the reagent without affection of the other ingredients contained in the sample.

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"Chemical Modification of the Nonreducing, Terminal Group of Maltotriose", Ken'Ichi Takeo, Toshinari Matsunami, and Takashi Kuge, Carbohydrate Research, vol. 51 (1976), pp. 73-84.

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