Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1992-06-05
1994-04-12
Griffin, Ronald W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
536 41, 536 173, 536 175, 536 177, 536 184, 536 187, 53612313, 435 96, 435 98, 435 99, C12Q 140
Patent
active
053025148
ABSTRACT:
The present invention provides a maltooligoside derivative represented by the formula: ##STR1## wherein n denotes an integer 3-5, R represents an aromatic chromophoric group, X represents a group >CHCH.sub.2 N.sub.3 or >C.dbd.CH.sub.2, and Y represents a hydrogen atom, a substituted or unsubstituted hydrocarbon group or an alkyl- or arylsulfonyl group, a reagent for determining .alpha.-amylase activity which comprises said maltooligoside derivative as an effective ingredient, and a process for determining .alpha.-amylase activity, characterized in that said maltooligoside derivative and coupled enzymes are added to an .alpha.-amylase containing sample to conduct an enzymatic reaction and a liberated aromatic chromophoric compound is quantitatively determined. The compound of the present invention is very useful as a substrate for determining .alpha.-amylase activity, and .alpha.-amylase activity can be easily measured accurately in a short time by using the reagent without affection of the other ingredients contained in the sample.
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Kotani Kazuo
Saito Kazunori
Tobe Kohichiro
Tokutake Shoichi
Tomikura Tadashi
Daiichi Pure Chemicals Co. Ltd.
Griffin Ronald W.
Kikkoman Corporation
Seishin Pharmaceutical Co., Ltd.
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