Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-03-01
1996-06-18
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435202, 435829, 4352521, C12N 926, C12N 928, C12N 112, C12N 100
Patent
active
055276997
ABSTRACT:
An amylase is preferrably obtained from Alcaligenes latus FERM BP-4578. The amylase has an activity of mainly forming maltohexaose and maltoheptaose from starch, but does not substantially have an activity of hydrolyzing maltohexaose and an oligosaccharide having a molecular weight lower than maltohexaose. The amylase does not substantially act on maltoheptaose and has a molecular weight of about 43,000+3,000 daltons as measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis. It has an isoelectric point of about 7.6+0.5 as measured on isoelectrophoresis using an ampholyte, an optimum pH of about 5.0 in the presence of calcium ion, an optimum temperature of about 70.degree. C. in the presence of calcium ion as measured in terms of relative enzyme activity, a pH stability of about 4.5-10.5 in the presence of calcium ion and a thermal stability up to about 60.degree. C. in the presence of calcium ion as measured in terms of residual enzyme activity. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich in maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.
REFERENCES:
Keiji Kainuma et al, Isolation and Action Pattern of Maltohexaose Producing Amylase From Aerobacter Aerogenes, FEBS Letter, vol 26, pp. 281-285, Oct. 1972.
J. F. Kennedy at al, Action Pattern and Substrate Specificity of alpha-Amylase K, a Novel Amylase from a Strain of Bacillus subtilis, Starch, vol. 31, pp. 235-241, 1979.
Yoshiyuki Takasaki, Production of Maltohexaose by .alpha.-Amylase from Bacillus ciruclans G-6, Agricultural and Biological Chemistry, vol. 46, pp. 1539-1547, 1982.
Hajime Taniguchi et al, Enzymic Digestion of Potato Starch Granules, J. Jap. Soc. Starch Science, vol. 29, pp. 107-116, 1982.
Iwao Maeda et al, Purification of R-enzyme from Malted Barley and Its Role in in vitro Digestion of Barley Starch Granules, J. Jap. Soc. Starch Science, vol. 26, pp. 117-127, 1979.
Iwao Maeda et al, Digestion of Barley Starch Granules by the Combined Action of .alpha.-and .beta.-Amylases Purified from Barley and Barley Malt, Agricultural and Biological Chemistry, vol. 42, pp. 259-267, 1978.
Takaya Hayashi et al, Production and Purification of New Maltohexaose-forming Amylases from Alkalophilic Bacillus sp. H-167, Agricultural and Biological Chemistry, vol. 52, pp. 443-448, 1988.
Chaen Hiroto
Miyake Toshio
Nakano Masayuki
Sugimoto Toshiyuki
Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
Meller Michael V.
Naff David M.
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