Maltohexaose and maltoheptaose-forming amylase, and its preparat

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435 99, 435202, 435829, 4352521, C12N 926, C12N 928, C12N 112, C12N 100

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active

057390240

ABSTRACT:
Disclosed is a novel amylase which mainly forms maltohexaose and maltoheptaose when acts on starch, but does not substantially hydrolyze maltohexaose and a lower molecular oligosaccharide than maltohexaose. The amylase can be prepared from microorganisms of the genus Alcaligenes, and has a relatively-high optimum temperature and thermal stability, as well as a relatively-wide range of optimum pH and pH stability. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich in maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.

REFERENCES:
ATCC Catalogue of Bacteria and Bacteriophages, 17th Ed., 1989, pp. 13, 14.
Keiji Kainuma et al, Isolation and Action Pattern of Maltohexaose Producing Amylase From Aerobacter Aerogenes, FEBS Letter, vol. 26, pp. 281-285, Oct. 1972.
J. F. Kennedy et al, Action Pattern and Substrate Specificity of alpha-Amylase K, a Novel Amylase from a Strain of Bacillus Subtilis, Starch, vol. 31, pp. 235-241, 1979.
Yoshiyuki Takasaki, Production of Maltohexaose by .alpha.-Amylase from Bacillus circulans G-6, Agricultural and Biological Chemistry, vol. 46, pp. 1539-1547, 1982.
Hajime Taniguchi et al, Enzymic Digestion of Potato Starch Granules, J. Jap. Soc. Starch Science, vol. 29, pp. 107-116, 1982.
Iwao Maeda et al, Purification of R-enzyme from Malted Barley and Its Role in in vitro Digestion of Barley Starch Granules, J. Jap. Soc. Starch Science, vol. 26, pp. 117-127, 1979.
Iwao Maeda et al, Digestion of Barley Starch Granules by the Combined Action of .alpha.-and .beta.-Amylases Purified from Barley and Barley Malt, Agricultural and Biological Chemistry, vol. 42, pp. 259-267, 1978.
Takaya Hayashi et al, Production and Purification of New Maltohexaose-forming Amylases from Alkalophilic Bacillus sp. H-167, Agricultural and Biological Chemistry, vol. 52, pp. 443-448, 1988.

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