Maltohexaose and maltoheptaose forming amylase, and its preparat

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435829, 435201, 435202, C12N 120, C12N 926, C12N 928

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active

058468080

ABSTRACT:
Disclosed is a novel amylase which mainly forms maltohexaose and maltoheptaose when reacted with starch, but does not substantially hydrolyze maltohexaose and an oligosaccharide of lower molecular than maltohexaose. The amylase can be prepared from microorganisms of the genus Alcaligenes, and has a relatively-high optimum temperature and thermal stability, as well as a relatively-wide range of optimum pH and pH stability. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich on maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.

REFERENCES:
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Kainuma et al, "Isolation and Action Pattern of Maltohexaose Producing Amylase from Aerobacter Aerogenes"; FEBS Letter, 26:281-285 (1972).
Kennedy et al, Action Pattern and Substrate Specificity of alpha-Amylase K, a Novel Aylase from a Strain of Bacillus Subtilis; Starch, 31:235-241 (1979).
Maeda et al, "Purification of R-enzyme from Malted Barley and Its Role in in vitro Digestion of Barley Starch Granules"; J. Jap. Soc. Starch Science, 26:117-127 (1979).
Maeda et al, "Digestion of Barley Starch Granules by the Combined Action of alpha-and beta-Amylases Purified fro mBarley and Barley Malt"; Agricultural and Biological Chemistry, 42:259-267 (1978).
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Taniguchi et al, "Enzymic Digestion of Potato Starch Granules"; J. Jap. Soc. Starch Science, 29:107-116 (1982).
CA 119: 27066p, A. Totsuka et al., "Maltooligosaccharide Compositions for Use as Sweetening Agents and Their Preparation from Starch with .alpha.-Amylase and Debranching Enzymes", Chemical Abstracts, vol. 119, No. 3, p. 77, Jul. 19, 1993.
CA 119: 48070a, A. Totsuka et al., "Reduced Starch Sugar Compositions Containing Maltohexitol and Meltoheptitol for use in Foods and Beverages", Chemical Abstracts, vol. 119, No. 5, Aug. 2, 1993.
I. Maeda et al., "Digestion of Barley Starch Granules by the Combined Action of Alpha-and Beta-Amylases Purifird Barley and Barley Malt", Agricultural Biological Chemical, vol. 42, pp. 259-267, 1978.
R.-Q. He et al., "An asynchronous unfolding among molecular different regions of lobster D-glyceraldehyde-3-phosphate dehydrogenase and maltotetrsose-forming amylase from an Alcaligenes sp. during guanidine denaturation", Biochimica et Biophysica Acta, vol. 1163, pp. 315-320, 1993 .

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