Malathion carboxylesterase

Details Malathion carboxylesterase Malathion carboxylesterase

2006-08-15

2006-08-15

Rao, Manjunath N. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S004000, C435S006120, C435S069100, C435S183000, C435S252300, C435S320100, C435S262000, C536S023200, C530S350000

Reexamination Certificate

active

07091025

ABSTRACT:
The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyloxon organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60%, preferably at least 80% and more preferably at least 95% homology with Lc?E 7, in which the protein encoded by the DNA molecule differs from E 3 at least in the substitution of Trip at position 251 with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.

REFERENCES:
patent: 14502/95 (1995-07-01), None
patent: WO 90/02177 (1990-03-01), None
patent: WO 95/19440 (1990-07-01), None
patent: 95/19440 (1995-07-01), None
“Insecticide Resitance and Malathion Carboxylesterase in the Sheep Blowfly, Lucitia Cuprina,” by Whyard et al., Biochemical Genetics, vol. 32, Nos. 1/2 (1994), pp. 9-24.
“Characterization of Malathion Carboxylesterase in the Sheep Blowfly Lucilia Cuprina,” by Whyard et al., Pesticide Biochemistry and Physiology, vol. 50, No. 3 (1994), pp. 198-206.
“Characterization of a Novel Esterase Conferring Insecticide Resistance in the Mosquito Culex Tarsalis,” by Whyard et al., Archives of Insect Biochemistry and Physiology, vol. 29 (1995), pp. 329-342.
“Isolation of an Esterase Conferring Insecticide Resistance in the Mosquito Culex Tarsalis,” by Whyard et al., Insect Biochem. Molec. Biol., vol. 24, No. 8 (1994), pp. 819-827).
Smyth et al. “A Cluster of at Least Three Esterase Genes inLucilia cuprinaIncludes Malathion Carboxylesterase and Two Other Esterases Implicated in Resistance to Organophosphates” Biochemical Genetics, vol. 32, No. 11-12, 1994, pp. 437-453, XP 002293390 ISSN: 0006-2928.
Campbell et al., “Two different amino acid substitutions in the all-esterase, E3, confer alternative types of organophosphorus insecticide resistance in the sheep blowfly,Lucilia cuprina”, Insect Biochemistry and Molecular Biology, vol. 28, No. 3, Mar. 1998 pp. 139-150, Xp 002293391, ISSN: 0965-1748.

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