Making nucleic acid sequences in parallel and use

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Reexamination Certificate

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07544793

ABSTRACT:
The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins.

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Barndad, Cynthia, “A DNA, Self-Assembled Monolayer for the Specific Attachment of Unmodified Double- or Single-Stranded DNA,” Biophysical Journal, Oct. 1998, vol. 75, pp. 1997-2003.
Zhou et al., “Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences,” Nucleic Acids Research, vol. 32, No. 18, pp. 5409-5417.
Sarkar, et al. Nucleic Acids Res. 2005. vol. 33, No. 1, pp. 143-151.

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